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| 益生菌Lactobacillus casei Zhang在酸胁迫下的蛋白质组学研究 | |
| 引用本文： | 乌日娜,岳喜庆,张和平. 益生菌Lactobacillus casei Zhang在酸胁迫下的蛋白质组学研究[J]. 食品与发酵工业, 2012, 0(7): 17-20 |
| 作者姓名： | 乌日娜 岳喜庆 张和平 |
| 作者单位： | 沈阳农业大学食品学院;内蒙古农业大学教育部乳品与生物技术重点实验室 |
| 基金项目： | 国家自然科学基金项目(3102501931000805);教育部创新团队发展计划(IRT0967);教育部创新团队发展计划(IRT0967);973计划前期研究专项基金项目(2010CB134502);“863”计划项目(2011AA100902);辽宁省高等学校优秀人才支持计划(LJQ2011071);沈阳农业大学“天柱山英才计划” |
| 摘 要： | Lactobacillus casei Zhang是1株筛选自发酵酸马奶,并具有耐酸等益生特性的益生乳杆菌。该研究利用蛋白质组双向电泳,比较了其在pH值为7.0和5.5的培养液中分别生长至对数生长期中期的蛋白质组表达差异。结果表明,有11个蛋白质点表达发生明显变化,经过基质辅助激光解吸/电离飞行时间质谱鉴定,其中有3个表达增强的蛋白质点分别鉴定为翻译因子(EF-Tu),N-乙酰氨基葡萄糖-6-磷酸脱醛酶(NagA)和小热休克蛋白(sHsp)。酸胁迫可诱导乳酸菌产生复杂的酸应激反应,涉及不同的代谢调控途径。 |
| 关 键 词： | Lactobacillus casei Zhang 蛋白质组 酸胁迫 |
Proteomics Researches on Acid Response of Lactobacillus casei Zhang Grown at Different pH Values Using Two-Dimensional Gel Electrophoresis |
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| Wu Ri-na,Yue Xi-qing,Zhang He-ping. Proteomics Researches on Acid Response of Lactobacillus casei Zhang Grown at Different pH Values Using Two-Dimensional Gel Electrophoresis[J]. Food and Fermentation Industries, 2012, 0(7): 17-20 | |
| Authors: | Wu Ri-na Yue Xi-qing Zhang He-ping |
| Affiliation: | 1(College of Food Science,Shenyang Agricultural University,Shenyang 110866,China) 2(Key Laboratory of Dairy Biotechnology and Bioengineering,Ministry of Education, Inner Mongolia Agricultural University,Hohhot 010018,China) |
| Abstract: | Lactobacillus casei Zhang,isolated from traditional home-made koumiss in Inner Mongolia of China,has been considered as a new probiotic bacterium for its characteristics,especially acid tolerance.Proteomics researches were carried out to identify proteins expression of Lactobacillus casei Zhang grown at different pH values of pH 7.0 and 5.5.Cytosolic proteins of mid-exponential phase were resolved and further analyzed by two-dimensional gel electrophoresis.As results shown,eleven protein spots were found showing statistically significant differences.Three of total protein spots were identified as EF-Tu,NagA and sHsp using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF/MS),indicating the complex response of lactic acid bacteria to acid stress. |
| Keywords: | Lactobacillus casei Zhang proteomics acid stress |
| 本文献已被 CNKI 等数据库收录！ | |