| 96孔板高通量选育产乙醇酵母 |
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| 引用本文： | 张继琳,张栩,谭天伟.96孔板高通量选育产乙醇酵母[J].酿酒,2007,34(1):71-73. |
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| 作者姓名： | 张继琳 张栩 谭天伟 |
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| 作者单位： | 北京化工大学生命科学与技术学院,北京市生物加工过程重点实验室,北京,100029 |
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| 摘 要： | 以巴斯德毕赤酵母(Pichia pastoris),安琪酵母(Angel yeast),酿酒酵母(Sacchro-myces cerevisiae)等4株菌为出发菌株,经过紫外诱变,96孔板高通量筛选出60株,复筛选出4株.最大乙醇产量提高到76.6g/L,糖醇转化率达到了0.488g/g.对上述4株菌进行耐乙醇性能驯化,发酵耐乙醇能力达到15%.
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| 关 键 词： | 乙醇发酵 葡萄糖 紫外诱变 96孔板筛选 孔板 高通量筛选 选育 乙醇 酿酒酵母 Screening High Producer Fuel Ethanol 能力 发酵 驯化 醇性能 醇转化率 产量提高 紫外诱变 菌株 Angel yeast 安琪酵母 |
| 文章编号： | 1002-8110（2007）01-0071-03 |
| 收稿时间： | 2006-09-25 |
| 修稿时间： | 2006年9月25日 |
Mutation and Screening of Fuel Ethanol Producer through 96-well Plantes High Through put Screening |
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| ZHANG Ji-lin,ZHANG Xu,TAN Tian-wei.Mutation and Screening of Fuel Ethanol Producer through 96-well Plantes High Through put Screening[J].Liquor Making,2007,34(1):71-73. |
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| Authors: | ZHANG Ji-lin ZHANG Xu TAN Tian-wei |
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| Affiliation: | Beijing Key Lab of Bioproeess, College of Life Sciences and Technology, Beijing University of Chemieal Technology, Beijing 100029,China |
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| Abstract: | From these yeast trains,which can be easy attained in own laboratory,60 strains were screened through UV mutation using 96-well plates high throughput screening(HTS).4 strains were selected after advanced screening.The maximal ethanol production was enhanced to 76.6g/L and the transform rate was 0.488g/g.Through domestication with ethanol addition before fermentation,the ethanol tolerance of these 4 strains was all above 8%.The tolerance before fermentation got to 12%,the final ethanol content in fermentation broth was 15%. |
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| Keywords: | ethanol fermentation glucose UV mutation 96-well plates HTS |
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