大孔树脂纯化红树莓籽黄酮及其体外结合胆酸盐能力评价

以红树莓籽黄酮为研究对象,考察了AB-8大孔树脂纯化黄酮最佳条件,并研究不同提取方法和纯化对产品胆酸盐结合能力的影响。结果表明:红树莓籽黄酮上样浓度10 mg/mL,AB-8大孔树脂最佳纯化条件为:上样量100 mL、pH4.5、上样流速1.5 mL/min,以60%乙醇、150 mL、流速1.5 mL/min洗脱,红树莓籽黄酮纯度可达48.19%,与未纯化相比提高了23.12%;醇提、超声波、酶提和AB-8纯化4种方式所得黄酮,对3种胆酸盐结合能力依次为:AB-8纯化、醇提、超声波和酶提黄酮,其中AB-8纯化黄酮对牛磺胆酸钠、甘氨胆酸钠、胆酸钠3种胆酸盐结合能力依次为79.35%、74.88%和74.56%,结果表明,红树莓籽黄酮对胆酸盐均有一定结合能力,其中AB-8纯化黄酮结合能力最强。

Purification of Flavonoids from Red Raspberry Seed by Macroporous Resin and the Ability of Bile Salt-binding in Vitro

The purification conditions of red raspberry seed flavonoids by AB-8 macroporous resin were investigated and its ability of binding bile salts was evaluated. The optimal purification conditions of AB-8 macroporous resin was as follows:Sample concentration was 10 mg/mL,sample volume was 100 mL,the sample flow rate was 1.5 mL/min and sample pH was 4.5,the volume fraction of the ethanol was 60%,the volume of elution was 150 mL,and the flow rate of elution was 1.5 mL/min. The purity of red raspberry seed flavonoids was 48.19% and which was 23.12% higher than those unpurified. And four kinds of extraction methods for the three kinds of bile salt binding ability was in the order:purified by AB-8,ethanol extracted,ultrasonic extracted and enzyme extracted flavonoids. Moreover,hypolipidemic experiments showed that purified flavonoids by AB-8 had strong combining capabilities for sodium taurocholate,sodium glycocholate,sodium cholate with combination rate of 79.35%,74.88% and 74.56%. The results showed that the red raspberry seeds flavonoids had ability to bind to bile salts,among which AB-8 had the strongest binding ability.