玉米肽Tyr-Phe-Cys-Leu-Thr对酒精性HepG2细胞的保护作用

以从玉米蛋白粉中鉴定得到的玉米肽Tyr-Phe-Cys-Leu-Thr(YFCLT)为研究对象,通过体外抗氧化试验和细胞模型试验,从氧化应激与脂质代谢两个方面探讨其对HepG2细胞酒精性损伤的保护作用。体外抗氧化试验结果表明:玉米肽Tyr-Phe-Cys-Leu-Thr在较低浓度1μmol/L时仍具有一定的DPPH自由基清除能力、ABTS自由基清除率、铁螯合能力和氧自由基吸收能力(ORAC)。采用酒精为诱导剂诱导建立人肝癌细胞(HepG2)酒精性损伤模型,酒精最佳作用浓度为500 mmol/L,作用24 h。HepG2细胞酒精性损伤模型试验结果表明:玉米肽Tyr-Phe-Cys-Leu-Thr具有通过降低模型细胞ALT、AST的泄漏量,降低SOD、CAT、GSH酶活力,降低ROS和细胞TNF-α释放量而起到抗氧化应激损伤的HepG2细胞保护作用。玉米肽Tyr-Phe-Cys-Leu-Thr也可降低HepG2细胞内TG含量及减弱脂过氧化。综合各指标,玉米肽Tyr-Phe-Cys-Leu-Thr对HepG2细胞酒精性损伤具有保护作用。

Protective Effect of Corn Peptide Tyr-Phe-Cys-Leu-Thr on Alcoholic HepG2 Cells

Tyr-Phe-Cys-Leu-Thr(YFCLT),a corn peptide identified from corn gluten meal,was used as the research object.Through the in vitro anti-oxidation test and cell model test,the protective effects on HepG2 cells were studied from two aspects of oxidative stress and lipid metabolism.The results of antioxidant test in vitro showed that Tyr-Phe-Cys-Leu-Thr had DPPH radical scavenging ability,ABTS radical scavenging rate,iron chelating ability and oxygen radical absorbing ability(ORAC)at a lower concentration of 1μmol/L.Alcohol was used as the inducer to induce the alcoholic injury model of human hepatocarcinoma cells(HepG2).The optimal concentration of alcohol was 500 mmol/L for 24 hours.The results showed that Tyr-Phe-Cys-Leu-Thr could protect the HepG2 cells from oxidative stress injury by reducing the leakage of ALT and AST,the activity of SOD,cat and GSH,and the release of ROS and TNF-α.Tyr-Phe-Cys-Leu-Thr can also reduce the content of TG in cells and the accumulation of fat,thus play a protective role in lipid metabolism.In conclusion,Tyr-Phe-Cys-Leu-Thr can protect HepG2 cells from alcohol induced injury.