| 银杏内酯A和B的分离纯化研究 |
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| 引用本文： | 韩金玉,王华,常贺英,褚巧伟.银杏内酯A和B的分离纯化研究[J].中国化学工程学报,2003,11(2):125-129. |
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| 作者姓名： | 韩金玉 王华 常贺英 褚巧伟 |
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| 作者单位： | SchoolofChemicalEngineeringandTechnology,TianjinUniversity,Tianjin300072,China |
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| 基金项目： | Supported by the Natural Science Foundation of Tianjin(No.993606911). |
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| 摘 要： | In this paper a simple preparative method for isolation and purification of ginkgolides A and B was developed,As starting material,a commercially available standardized ginkgo extract (EGb761,containing 24% flavonoid and 6% terpene trilactones) was used,After a pretreatment step,optimized by the uniform design method ,the concentrated intermediate extract with high content of GA and gb( 90%) was separated into the individual terpenes by preparative liquid chromatography eluted with petroleum ether-ethylacetate,Analysis of products was carried out by means of HPLC-ELSD(evaporative light -scattering detector),The results show that ginkgolides A and B are obtained in higher yield and better purity.
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| 关 键 词： | 纯化 银杏汁 分离 类黄酮 萜烯 提取 分析 生物活性 气相色谱 银杏内酯A 银杏内酯B |
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Isolation and Preparative Purification for Ginkgolides A and B |
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| HAN Jinyu,WANG Hua,CHANG Heying,CHU Qiaowei.Isolation and Preparative Purification for Ginkgolides A and B[J].Chinese Journal of Chemical Engineering,2003,11(2):125-129. |
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| Authors: | HAN Jinyu WANG Hua CHANG Heying CHU Qiaowei |
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| Affiliation: | School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
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| Abstract: | In this paper, a simple preparative method for isolation and purification of ginkgolides A and B was developed. As starting material, a commercially available standardized ginkgo extract (EGb761, containing 24% flavonoid and 6% terpene trilactones) was used. After a pretreatment step, optimized by the uniform design method, the concentrated intermediate extract with high content of GA and GB (+90%) was separated into the individual terpenes by preparative liquid chromatography eluted with petroleum ether-ethylacetate. Analysis of products was carried out by means of HPLC-ELSD (evaporative light-scattering detector). The results show that ginkgolides A and B are obtained in higher yield and better purity. |
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| Keywords: | ginkgolide A ginkgolide B isolation and purification uniform design preparative liquid chromatog-raphy |
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