| 咖啡因联合辐射处理对人乳腺癌易感基因BRCA1表达的影响 |
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| 引用本文： | 李宁,张红,王燕玲,周鑫,郝冀方.咖啡因联合辐射处理对人乳腺癌易感基因BRCA1表达的影响[J].原子能科学技术,2011,45(1):124-128. |
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| 作者姓名： | 李宁 张红 王燕玲 周鑫 郝冀方 |
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| 作者单位： | 1.中国科学院 ;近代物理研究所，甘肃 ;兰州730000；2.中国科学院 ;重离子束辐射生物医学重点实验室，甘肃 ;兰州730000；3.甘肃省重离子束辐射医学应用基础重点实验室，甘肃 ;兰州730000；4.中国科学院 ;研究生院，北京100049 |
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| 基金项目： | 国家自然科学基金资助项目，甘肃省中青年科技基金资助项目 |
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| 摘 要： | 研究了咖啡因对碳离子束辐射的增敏效应，并观察在该过程中人乳腺癌易感基因BRCA1表达的变化。碳离子束联合咖啡因处理人乳腺癌细胞MCF-7后，利用实时细胞检测仪观察细胞生长、流式细胞术检测细胞周期分布变化、real-timePCR检测BRCA1mRNA水平、Westernblot检测BRCA1蛋白水平及其磷酸化水平。结果显示：咖啡因处理导致细胞生长明显受到抑制；咖啡因废除了辐射诱导的G2期阻滞，抑制了BRCA1mRNA和蛋白表达水平，同时特异性抑制了BRCA1的丝氨酸1524位点的磷酸化作用。这些结果表明咖啡因抑制了BRCA1蛋白和其磷酸化表达水平。
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| 关 键 词： | BRCA1 离子辐射 咖啡因 辐射敏感性 G2期检测点 |
Effects of Caffeine Cotreatment With Radiation on Breast Cancer Susceptibility Gene BRCA1 |
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| LI Ning,ZHANG Hong,WANG Yan-ling,ZHOU Xin,HAO Ji-fang.Effects of Caffeine Cotreatment With Radiation on Breast Cancer Susceptibility Gene BRCA1[J].Atomic Energy Science and Technology,2011,45(1):124-128. |
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| Authors: | LI Ning ZHANG Hong WANG Yan-ling ZHOU Xin HAO Ji-fang |
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| Affiliation: | 1.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;2.Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China;3.Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China;4.Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | The sensitizing effect of caffeine to carbon ion radiation was investigated and the change of BRCA1 expression was observed.The MCF-7 breast carcinoma cells were exposed to carbon ion beams with or without caffeine.The cell survival was automatically monitored by RT-CES system.Cell cycle distribution was assessed by flow cytometry.The levels of BRCA1 mRNA were analyzed by real-time RT-PCR.The expression of BRCA1 protein and its phosphorylation were examined by Western blot.The results show that caffeine inc... |
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| Keywords: | BRCA1 |
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