Thermoresponsive poly(<Emphasis Type="Italic">N</Emphasis>-vinylcaprolactam) cryogels: synthesis and its biophysical evaluation for tissue engineering applications |
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Authors: | Akshay Srivastava Ashok Kumar |
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Affiliation: | (1) Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, 208016 Kanpur, India; |
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Abstract: | The thermoresponsive poly(N-vinylcaprolactam) (PVCl) based cryogel network were synthesized and characterized with respect to physical and biological
properties. The PVCl cryogel crosslinked with polyethylene glycol-diacrylate (PEGda) was synthesized in 1% dimethyl sulfoxide
containing aqueous medium at −12°C for 12–14 h. The cryogel synthesized in this manner were highly spongy in nature and can
absorb water in its porous network. These polymeric cryogel networks have good physical morphology as confirmed by scanning
electron microscopy. The estimated porosity of these cryogels was 90% as demonstrated by various methods based on absorption
of water and cyclohexane. The median pore diameter and surface area was 30 μm and 2.0253 m2/g, respectively as confirmed by analysis on mercury porosimeter. These materials can interact with biological system without
any cytotoxic effects. Change in temperature influenced the adsorption of fetal bovine serum (FBS) on PVCl scaffold which
showed maximum protein adsorption at 37°C, as compared to that at 25°C. Furthermore, the fibroblast cell adhesion studies
showed the potential of these PVCl based cryogels as tissue engineering scaffolds. |
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