| 产黄青霉β-甘露聚糖酶的高效表达、性质及应用 |
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| 引用本文： | 甄红敏,华晓晗,马俊文,温永平,李延啸,闫巧娟,江正强.产黄青霉β-甘露聚糖酶的高效表达、性质及应用[J].食品科学,2021,42(8):98-105. |
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| 作者姓名： | 甄红敏 华晓晗 马俊文 温永平 李延啸 闫巧娟 江正强 |
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| 作者单位： | （1.中国农业大学食品科学与营养工程学院，北京 100083；2.中国农业大学工学院，北京 100083；3.蒙牛高科乳制品（北京）有限责任公司，北京 100101） |
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| 基金项目： | 国家自然科学基金青年科学基金项目（31901627）；中国博士后科学基金面上项目（2018M641539） |
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| 摘 要： | 将产黄青霉（Penicillium chrysogenum）来源的β-甘露聚糖酶（PcMan26A）在毕赤酵母中高效表达，经高密度发酵，发酵液酶活力达25 200 U/mL。该酶属于GH26家族，与黑曲霉（Aspergillus niger）CBS 513.88来源的β-甘露聚糖酶同源性最高（67.8%），是一个新型β-甘露聚糖酶。PcMan26A的最适催化条件为pH 6.0和50?℃，在pH?4.0～8.0和45?℃下具有良好的稳定性。该酶对魔芋粉具有最高的比活力，为3?581.0?U/mg。进一步利用该酶水解魔芋粉得到魔芋甘露寡糖，产品得率为86.2%；经分析，其主要组分为聚合度大于4的甘露寡糖。该β-甘露聚糖酶适用于生产魔芋甘露寡糖，为魔芋甘露寡糖的酶法生产提供了更多的选择。
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| 关 键 词： | 产黄青霉 β-甘露聚糖酶 毕赤酵母 魔芋粉 甘露寡糖 |
High-level Expression,Characterization, and Application of a Novel β-Mannanase from Penicillium chrysogenum |
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| ZHEN Hongmin,HUA Xiaohan,MA Junwen,WEN Yongping,LI Yanxiao,YAN Qiaojuan,JIANG Zhengqiang.High-level Expression,Characterization, and Application of a Novel β-Mannanase from Penicillium chrysogenum[J].Food Science,2021,42(8):98-105. |
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| Authors: | ZHEN Hongmin HUA Xiaohan MA Junwen WEN Yongping LI Yanxiao YAN Qiaojuan JIANG Zhengqiang |
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| Affiliation: | (1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2. College of Engineering, China Agricultural University, Beijing 100083, China; 3. Meng Niu Hi-Tech Dairy (Beijing) Co. Ltd., Beijing 100101, China) |
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| Abstract: | A cloned β-mannanase (PcMan26A) gene from Penicillium chrysogenum was successfully expressed in Penicillium chrysogenum. At the end of high cell density fermentation, the enzymatic activity of the fermentation supernatant reached 25 200 U/mL. The enzyme belonged to the glycoside hydrolase family 26 and shared the highest amino acid sequence identity (67.8%) with β-mannanase from Aspergillus niger CBS 513.88. The optimal reaction conditions for PcMan26A were pH 6.0 and 50 ℃, and it was stable at 45 ℃ and within a broad pH range of 4.0–8.0. The enzyme showed the highest specific activity (3 581.0 U/mg) towards konjac powder. Furthermore, PcMan26A was used for konjac powder hydrolysis, yielding konjac manno-oligosaccharide with a yield of 86.2%. The main composition of the konjac manno-oligosaccharide was manno-oligosaccharides with degree of polymerization > 4. The recombinant β-mannanase provides a new option for the enzymatic production of konjac manno-oligosaccharide. |
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| Keywords: | Penicillium chrysogenum β-mannanase Pichia pastoris konjac powder manno-oligosaccharide |
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