| 基于单克隆抗体的胶体金免疫层析法快速检测丁香酚 |
| |
| 引用本文： | 解超男,李 芹,韩 刚,刘 欢,吴立冬,李晋成.基于单克隆抗体的胶体金免疫层析法快速检测丁香酚[J].食品安全质量检测技术,2019,10(20):6938-6943. |
| |
| 作者姓名： | 解超男 李 芹 韩 刚 刘 欢 吴立冬 李晋成 |
| |
| 作者单位： | 中国水产科学研究院, 质量与标准研究中心,中国水产科学研究院, 质量与标准研究中心,中国水产科学研究院, 质量与标准研究中心,中国水产科学研究院, 质量与标准研究中心,中国水产科学研究院, 质量与标准研究中心,中国水产科学研究院, 质量与标准研究中心 |
| |
| 基金项目： | 中国水产科学研究院院部中央级公益性科研院所基本科研业务费专项资金项目(2018C005) |
| |
| 摘 要： | 目的 采用免疫层析技术对丁香酚残留的胶体金免疫层析快速检测试纸条的制备开展研究。方法 用柠檬酸三钠还原法制备胶体金纳米颗粒, 标记丁香酚单克隆抗体得到胶体金-丁香酚单克隆抗体复合物。以硝化纤维素膜为固相载体, 包被丁香酚-BSA偶联物为检测带(T带), 羊抗鼠IgG为质控带(C带), 建立了丁香酚的胶体金免疫层析快速检测试纸条。结果 胶体金免疫层析试纸条具有较高的灵敏度和特异性, 检出限为2.0 mg/L。结论 本研究所开发的胶体金免疫层析试纸条可作为快速测定丁香酚的可靠、低成本的方法。
|
| 关 键 词： | 丁香酚 快速检测 胶体金免疫 |
| 收稿时间： | 2019/6/26 0:00:00 |
| 修稿时间： | 2019/10/23 0:00:00 |
Rapid detection of eugenol using colloidal gold immunoassay based on monoclonal antibody |
| |
| XIE Chao-Nan,LI Qin,HAN Gang,LIU Huan,WU Li-Dong and LI Jin-Cheng.Rapid detection of eugenol using colloidal gold immunoassay based on monoclonal antibody[J].Food Safety and Quality Detection Technology,2019,10(20):6938-6943. |
| |
| Authors: | XIE Chao-Nan LI Qin HAN Gang LIU Huan WU Li-Dong and LI Jin-Cheng |
| |
| Affiliation: | Quality and Standard Research Center, Chinese Academy of Fishery Sciences,Quality and Standard Research Center, Chinese Academy of Fishery Sciences,Quality and Standard Research Center, Chinese Academy of Fishery Sciences,Quality and Standard Research Center, Chinese Academy of Fishery Sciences,Quality and Standard Research Center, Chinese Academy of Fishery Sciences and Quality and Standard Research Center, Chinese Academy of Fishery Sciences |
| |
| Abstract: | Objective To study the preparation of colloidal gold immunochromatographic strip for rapid detection of eugenol residue by immunochromatographic technique. Methods Colloidal gold nanoparticles were prepared by citrate reduction method, and colloidal gold-eugenol monoclonal antibodies conjugates were prepared. Colloidal gold immunochromatographic strips for rapid detection of eugenol was established by using nitrocellulose membrane as solid carrier, eugenol-BSA conjugate as the detection line (T line) and goat-anti-rat IgG as the control line (C line). Results The developed strip showed high sensitivity and specificity, detection limit was 2.0 mg/L. Conclusion The described colloidal gold immunoassay strip can be used as a reliable and low cost method for the rapid determination of eugenol. |
| |
| Keywords: | eugenol rapid detection colloidal gold immunoassay |
|
| 点击此处可从《食品安全质量检测技术》浏览原始摘要信息 |
|
点击此处可从《食品安全质量检测技术》下载全文 |
|
