2016年江苏省淡水养殖环节常见致病性弧菌污染状况调查

目的 了解江苏省淡水动物性水产品养殖环节中常见弧菌(副溶血性弧菌、霍乱弧菌等)的污染程度。 方法 参照《2016年国家食品安全风险监测淡水动物性水产品养殖环节中常见弧菌专项监测工作手册》进行样品的采集和检测。 结果 从淡水养殖场中采集水产品、水体、水底沉积物样品共507份, 检出副溶血性弧菌17株(3.4%); 霍乱弧菌4株(0.8%); 溶藻弧菌及创伤弧菌均未检出。检出的副溶血性弧菌均不携带毒力基因(trh-;tdh-), 主要血清型为O5:H7(41.2%), 主要耐受的抗生素为头孢唑林(94.1%), 其中3株PFGE带型相似性系数100%; 4株霍乱弧菌均为非O1/O139群, 未发现耐药株。结论 江苏省淡水养殖环节中副溶血性弧菌环境污染程度低, 但在淡水养殖环节中检出致病性弧菌, 提示淡食用水产品依然可能存在引发食物中毒的风险。

Contamination investigation of common pathogenic Vibrio species in freshwater aquaculture processing in Jiangsu province in 2016

Objective To investigate the contamination profile of common pathogenic Vibrio species (Vibrio parahaemolyticus, Vibrio cholerae, etc.) in freshwater aquaculture of Jiangsu province. Methods Samples were collected and detected according to the common Vibrio special monitoring work manual from 2016 National food safety risk monitoring - Freshwater animal aquatic products breeding. Results A total of 507 samples including freshwater products, aquaculture water and silt were collected from freshwater aquaculture farms. Vibrio parahaemolyticus was detected in 17 (3.4%) samples, while Vibrio cholera was detected in 4 (0.8%) samples. Neither Vibrio alginolyticus nor Vibrio vulnificus were isolated. The Vibrio parahaemolyticus strains were negative for both trh and tdh genes, the main serotype of which was O5:H7 (41.2%), and the resistance rate to cefazolin was 94.1%. The PFGE band pattern similarity rate of 3 strains among them were 100%. The Vibrio cholerae strains were all non-O1/O139, and no strains were found to be antibiotic resistant. Conclusion The freshwater cultivation environment has low Vibrio parahaemolyticus contamination, but the detection of pathogenic Vibrio species suggests freshwater products still may have risk to cause food poisoning, and provide data support for food safety risk assessment and traceability of freshwater products related foodborne diseases.