反相微乳液聚合荧光高分子F-PAM

通过Span80-Tween80/异辛烷/FM(4-甲氧基-N-（2-N′，N′-二甲基氨基乙基）萘二甲酰亚胺烯丙基氯化铵）-AM（丙烯酰胺）-H₂O反相微乳液聚合，制备了AM和FM的荧光高分子聚合物（F-PAM）.采用透射电镜（TEM）、原子力显微镜（AFM）、激光纳米粒度仪、荧光分光光度计等测试手段，测定了荧光高分子聚合物（F-PAM）的粒子形态、粒径和粒径分布等微观结构及其荧光性能.试验结果表明：所制备的F-PAM为单分散、球形的纳米材料，平均粒径（D）在60～145 nm之间;F-PAM的激发波长和发射波长分别为381 nm和462 nm.F-PAM激发光谱与发射光谱呈较好的镜像对称关系;F-PAM的相对荧光强度随着其质量浓度的增加而增加，且F-PAM的浓度与其荧光强度呈很好的线性关系，其线性相关系数（R）为0.9953，F-PAM的检测下限为1.83 mg·L^-1.

Properties of fluorescent polymer F-PAM prepared by reverse microemulsion polymerization

A fluorescent polymer F-PAM was prepared by the reverse microemulsion polymerization of Span80-Tween80/isooctane/FM［4-methoxy-N-(2-N′,N′-dimethylaminoethyl)naphthalimide allyl chloride quaternary ammonium salt］-AM(acrylamide)-H₂O.The micro-structure，fluorescence property and size distribution of F-PAM particle were studied by using TEM，AFM，laser nanometer measurement and fluorescence spectrophotometer.The results showed that the polymer F-PAM was a mono-disperse，globular nanometer material，and the average diameter of PAM particles（D）was 60～145 nm.The wavelength of the excited and the emitted spectrum of F-PAM were 381 nm and 462 nm,respectively，and the shapes were symmetric as mirror images.Fluorescence intensity increased with the concentration of F-PAM，and the relationship of fluorescence intensity and concentration of F-PAM was linear.The correlation coefficient（R）was 0.9953.The lower limit of detection was 1.83 mg·L^-1.