气相色谱-质谱与嗅觉测量法联用分析橙油中致香物质

采用气相色谱-质谱与嗅觉测量法(gas chromatography-mass spectrometry/olfactometry,GC-MS/O)联用的分析方法,对橙油的致香物质进行了分析与鉴别。GC-MS检测到35种挥发性物质,其中相对质量分数较高的化合物为柠檬烯(80.79%)、芳樟醇(3.85%)、癸醛(2.90%)、β-月桂烯(1.94%)、α-蒎烯(0.82%)、辛醛(0.71%)等。而GC-O分析到的具有香气的化合物为α-蒎烯、辛醛、柠檬烯、芳樟醇、α-松油醇、癸醛、肉豆蔻醛、(E,E)-2,4-癸二烯醛及一种具有花香、甜橘香的未知物质。采用强度法与稀释法相结合鉴别了橙油中的关键致香物质,结果表明,α-松油醇和未知物质具有最大的香气强度和稀释因子(FD因子),即二者对橙油整体香气贡献最大。采用GC-MS/O联用技术可以有效筛选出精油中的香味活性物质,而强度法与稀释法结合又可快速、准确、全面地分析样品中关键致香物质。

Identification of aromatic compounds in orange oil with gas chromatography-mass spectrometry/olfactometry

Gas chromatography with simultaneous mass spectrometry and olfactometry(GC-MS/O) was used to determine the aroma compounds in orange oil.Totally 35 volatile compounds were detected by GC-MS,mainly including limonene(80.79%),linalool(3.85%),decanal(2.90%),β-myrcene(1.94%),α-pinene(0.82%),octanal(0.71%).A total of 8 aroma-active compounds have been perceived,which were α-pinene,octanal,limonene,linalool,α-terpineol,decanal,tetradecanal,(E,E)-2,4-decadienal and one unknown compound.A combined method of direct intensity and aroma extract dilution analysis(AEDA) was employed to further detect and identify the aroma compounds in orange oil,which demonstrated that both α-terpineol and the unknown compound had the biggest FD factor and maximum aroma intensity and contributed most to the aroma of orange oil.It’s proved that GC-MS/O can filter out aroma compounds effectively,and the combination of direct intensity and aroma extract dilution analysis(AEDA) can help to distinguish the key aroma compounds quickly,accurately and comprehensively.