| 氯诺昔康与牛血清白蛋白相互作用的光谱法研究 |
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| 引用本文： | 金迎春,敖登高娃. 氯诺昔康与牛血清白蛋白相互作用的光谱法研究[J]. 化学试剂, 2011, 0(8): 680-684 |
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| 作者姓名： | 金迎春 敖登高娃 |
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| 作者单位： | 内蒙古大学化学化工学院; |
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| 基金项目： | 内蒙古自然科学基金资助项目(2009MS0206) |
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| 摘 要： | 在模拟人体生理条件下,用荧光光谱法和紫外-可见吸收光谱法研究氯诺昔康(LNXC)和牛血清白蛋白(BSA)结合反应的特征.研究表明:氯诺昔康与牛血清白蛋白形成复合物,从而猝灭牛血清白蛋白的内源性荧光,该过程为静态猝灭过程.根据Stern-Volmer方程求出不同温度下结合位点数和结合常数；根据Forster非辐射能量转移...
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| 关 键 词： | 氯诺昔康 牛血清白蛋白 相互作用 荧光光谱 紫外光谱 |
Spectroscopic method studies on the interaction between lornoxicam and bovine serum albumin |
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| JIN Ying-chun,AODENG Gao-wa. Spectroscopic method studies on the interaction between lornoxicam and bovine serum albumin[J]. Chemical Reagents, 2011, 0(8): 680-684 |
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| Authors: | JIN Ying-chun AODENG Gao-wa |
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| Affiliation: | JIN Ying-chun,AODENG Gao-wa(School of Chemistry and Chemical Engineering,Inner Mongolia University,Hohhot 010021,China) |
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| Abstract: | The interaction between lornoxicam and bovine serum albumin was studied at pH 7.0 by spectroscopic methods including fluorescence spectra and UV-visible absorption spectra.It showed that the complex formation between bovine serum albumin and lornoxicam resulted in the quenching of the intrinsic fluorescence of bovine serum albumin;The main mechanism of protein fluorescence quenching was a static quenching procedure.The binding site number n and apparent binding constant KA were measured at different tempera... |
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| Keywords: | lornoxicam bovine serum albumin interaction fluorescence spectroscopy UV spectroscopy |
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