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New hydrogels based on maleilated collagen with potential applications in tissue engineering

Authors:	Simona PotoracMarcel Popa Vasilica MaierGabriela Lisa Liliana Verestiuc

Affiliation:	^a “Gheorghe Asachi” Technical University, Faculty of Chemical Engineering and Environmental Protection, Department of Natural and Synthetic Polymers, 71 Dimitrie Mangeron, 700050 Iasi, Romania ^b “Gheorghe Asachi” Technical University, Faculty of Textile, Leather and Industrial Management, Department of Chemical Technology of Leather and Substitutes, 71 Dimitrie Mangeron, 700050, Iasi, Romania ^c “Gr.T.Popa” University of Medicine and Pharmacy, Faculty of Medical Bioengineering, Department of Biological Sciences, 9-13 Kogalniceanu Street, 700454, Iasi, Romania

Abstract:	New hydrogels based on maleic anhydride (MA) modified collagen were prepared with the aim of overcoming the high degradation rate displayed by collagen that is not otherwise chemically crosslinked. Semi-interpenetrated matrices were obtained by free radical polymerization of maleilated collagen (CM) and 2-hydroxyethyl methacrylate (HEMA) in the presence of ammonium persulfate (APS) and N,N,N′,N′-tetramethylethylenediamine (TEMED) as initiating system. The resulting matrices (CMH) had a sharp decrease in degradation, when compared to pure collagen. FTIR and H¹ NMR spectroscopies were used to confirm the incorporation of MA on the collagen peptide chains. The final composition of CMH was found to be strongly dependent by the concentration of maleilated collagen. The morphology of the hydrogels was studied by Scanning electron microscopy (SEM) and the macro-gel structure was confirmed. Water uptake of the synthetised hydrogels is influenced by both composition and the porosity of the matrices.

Keywords:	C native collagen MA maleic anhydride CM maleilated collagen CMH hydrogels based on maleilated collagen and HEMA HEMA 2-hydroxy ethyl methacrylate pHEMA poly(2-hydroxy ethyl methacrylate) APS ammonium persulfate TEMED N N N&prime N&prime -tetramethylethylenediamine DMSO dimethyl sulfoxide 15 8CM2APS collagen hydrogel with CM:HEMA = 15 8:84 2 and 2% APS (APS:HEMA %mol:mol) 44 1CM2APS collagen hydrogel with CM:HEMA = 44 1:55 9 and 2% APS (APS:HEMA %mol:mol) 30CM2APS collagen hydrogel with CM:HEMA = 30:70 and 2% APS (APS:HEMA %mol:mol) SIPN semi-interpenetrating polymer network PBS phosphate buffered saline TMS tetramethylsilane WU water uptake w_t weight at time point t w₀ initial sample weight DD degradation degree N_h concentration of α-NH₂ groups (mmol/ml × 10^&minus 10) for the obtained hydrogels N_C concentration of α-NH₂ groups (mmol/ml × 10^&minus 10) for pure collagen a₀ regression coefficient x_i x_j input variables Y response variable COR correlation coefficients ASE assymetric errors
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