| 微小毛霉凝乳酶基因的克隆与表达 |
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| 引用本文： | 姜媛媛,王景会,李玉秋,李达,杨贞耐. 微小毛霉凝乳酶基因的克隆与表达[J]. 中国乳品工业, 2010, 38(2) |
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| 作者姓名： | 姜媛媛 王景会 李玉秋 李达 杨贞耐 |
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| 作者单位： | 中国农业科技东北创新中心农产品加工研究中心,长春,130124 |
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| 基金项目： | 吉林省科学技术厅资助项目，国家863计划探索导向项目 |
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| 摘 要： | 微小毛霉(Mucor pusillus)凝乳酶是微生物凝乳酶的主要来源之一,但与传统的牛凝乳酶比较具有一定的缺陷。为将其采用基因工程的方法进行改造获得理想的凝乳酶,本研究克隆到微小毛霉凝乳酶基因,将其插入原核表达载体pTWlN1中,使之与几丁质结合域(CBD)一内含肽(intein)融合,获得原核表达质粒pTWIN1/M。转化大肠杆菌BL21(DE3),后经IPTG诱导后进行SDS-PAGE电泳分析,获得了重组蛋白。
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| 关 键 词： | 微小毛霉 克隆 表达 |
Cloning and expression of a rennet gene from mucor pusillus |
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| JIANG Yuan-yuan,WANG Jing-hui,LI Yu-qiu,LI Da,YANG Zhen-nai. Cloning and expression of a rennet gene from mucor pusillus[J]. China Dairy Industry, 2010, 38(2) |
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| Authors: | JIANG Yuan-yuan WANG Jing-hui LI Yu-qiu LI Da YANG Zhen-nai |
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| Affiliation: | JIANG Yuan-yuan,WANG Jing-hui,LI Yu-qiu,LI Da,YANG Zhen-nai(Center of Agro-food Technology,Northeast Agricultural Research Center of China,Changchun 130124,China) |
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| Abstract: | The rennet from Mucor pusillus is one of the major sources of microbial rennet.but it hassome defects comparing with the bovine chy-mosin.In order to develop a rennet with suitable milk clotting properties,the gene of rennetwas cloned from Mucor pusillus,and the DNA frag-ment was inserted into the prokaryotic expression vector pTWIN1,forming pTWIN1/M with a fusion protein integrated with(CBD-intein).pTWIN1/M was further transformed into E.coli BL21(DE3).Expression of the enzyme was performed with IPTG induc... |
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| Keywords: | mucor pusillus cloning expression |
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