| 大团囊虫草L2分离鉴定及其发酵工艺优化 |
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| 引用本文： | 许勤勤,吕龙贤,陈少云,郑静,郑高利,李永泉. 大团囊虫草L2分离鉴定及其发酵工艺优化[J]. 中国化学工程学报, 2009, 17(2): 278-285. DOI: 10.1016/S1004-9541(08)60206-2 |
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| 作者姓名： | 许勤勤 吕龙贤 陈少云 郑静 郑高利 李永泉 |
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| 作者单位： | 1. College of Life Sciences, Zhejiang University, Hangzhou 310058, China;2. Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China |
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| 基金项目： | China，国家高技术研究发展计划(863计划)，浙江省自然科学基金 |
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| 摘 要： | A strain isolated from the fruiting body of a fungus parasitized on Elaphomyces was identified as Cordyceps ophioglossoides based on the morphological characteristics and the analysis of ITS-5.8s rDNA sequence. The optimal medium composition (g•L－1), containing sucrose 66.0, yeast powder 10.0, silkworm chrysalises digest 30.0, MgSO4•7H2O 0.4, and KH2PO4 0.4, was found using fractional factorial design and a central composite design, and the optimization of cultural conditions obtained a result of seed age 6 days, inoculum size 6% (by volume), initial pH 5.6, temperature 24°C, shaking speed 160 r•min－1 by one-factor-at-a-time method. The maximum biomass reached about 20.2 g•L－1 after 90 hours culture under the optimal conditions. Elementary pharmacological activities showed that mycelia of C. ophioglossoides L2 from submerged culture promoted uterus growth in estrogen- depleted mice. In the 15-litre scale-up fermentation, the mycelial biomass was around 19.1 g•L－1, indicating a promising prospect for this biotechnology and the potency to develop its medical value.
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| 关 键 词： | Cordyceps ophioglossoides identification fermentation optimization fractional factorial design central composite design |
| 收稿时间： | 2008-06-23 |
| 修稿时间： | 2008-6-23 |
Isolation of cordyceps ophiogiossoides l2 from fruit body and optimization of fermentation conditions for its mycelial growth |
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| XU Qinqin,L Longxian,CHEN Shaoyun,ZHENG Jing,ZHENG Gaoli,LI Yongquan. Isolation of cordyceps ophiogiossoides l2 from fruit body and optimization of fermentation conditions for its mycelial growth[J]. Chinese Journal of Chemical Engineering, 2009, 17(2): 278-285. DOI: 10.1016/S1004-9541(08)60206-2 |
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| Authors: | XU Qinqin L Longxian CHEN Shaoyun ZHENG Jing ZHENG Gaoli LI Yongquan |
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| Affiliation: | 1. College of Life Sciences, Zhejiang University, Hangzhou 310058, China;2. Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China |
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| Abstract: | A strain isolated from the fruiting body of a fungus parasitized on Elaphomyces was identified as Cordyceps ophioglossoides based on the morphological characteristics and the analysis of ITS.5.8s rDNA sequence.30.0,MgSO4.7H2O 0.4,and KH,PO4 0.4,Was found using fractional factoriaI design and a central composite design,and the optimization of cultural conditions obtained a result of seed age 6 days,inoculum size 6%(by volume),initial pH 5.6,temperature 24°C,shaking speed 160 r-min-1 by one-factor-at.a.time method.The maximum biomass showed that mycelia of C.ophioglossoides L2 from submerged culture promoted uterus growth in estrogendepleted mice.In the 15-litre scale-up fermentation,the mycelial biomass was around 19.1 g.L-1,indicating a promising prospect for this biotechnology and the potency to develop its medical value. |
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| Keywords: | Cordyceps ophioglossoides identification fermentation optimization fractional factorial design central composite design |
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