Co-precipitation of whey and pea protein – indication of interactions |
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Authors: | Heidi Thorgaard Kristensen Anders Hauer Møller Mette Christensen Mikka Stenholdt Hansen Marianne Hammershøj Trine Kastrup Dalsgaard |
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Affiliation: | 1. Department of Food Science, Aarhus University, Agro Food Park 48, 8200 Aarhus N, Denmark;2. Arla Innovation Centre, Arla Foods Amba, Agro Food Park 19, 8200 Aarhus N, Denmark |
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Abstract: | The aim was to optimise the yield of co-precipitation of whey protein isolate (WPI) and pea protein isolate (PPI) and compare co-precipitates and protein blends with respect to solubility. The yield of co-precipitates was tested with different protein ratios of WPI and PPI in combination with different temperatures and acid precipitation (pH 4.6). The highest precipitation yield was obtained at protein ratios WPI < PPI, high temperature and alkaline protein solvation. The solubility was measured by an instability index and absorption spectroscopy of re-suspended precipitated proteins at pH 3, 7 and 11.5. Co-precipitates had significantly lower solubility than protein blends. Protein ratios WPI > PPI, low precipitation temperature and high pH showed the highest solubility. Differences in protein composition between co-precipitates and protein blends were observed with SDS-PAGE and matrix-assisted laser desorption ionisation time-of-flight, and indicated different protein–protein interaction in samples, which needs further investigations. |
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Keywords: | Co-precipitation optimised protein yield pea protein protein interactions protein solubility whey protein |
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