| Affiliation: | 1. State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi, 214122 China
School of Food Science and Technology, Jiangnan University, Wuxi, 214122 China;2. State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi, 214122 China;3. Jiangsu JUNQI Grain and Oil Limited Co., LTD, Nantong, 226000 China;4. School of Life Sciences, Yantai University, Yantai, Shandong, 264000 China |
| Abstract: | A two-stage microfiltration and ultrafiltration (MF/UF) for peanut protein recovery and water recycling from aqueous extraction processing (AEP) were investigated. Peanut protein aggregates and alkaline water were obtained using flat and wound MF/UF and alkaline water recycled in AEP. With this approach, most of the wastewater could be recycled to produce reusable water to solve wastewater problems in aqueous oil extraction processing from oilseeds. The result showed that a little oil and protein were lost (1.21% and 4.35%, respectively) during the recycling of permeate in AEP, which was still acceptable. Peanut protein aggregations (PPAs) were characterised by analysis with Fourier-transform infrared spectroscopy (FTIR), sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE), high-performance gel filtration chromatography (HPGFC) and binding force analysis. Solubility analysis suggested that PPA had molecular structures with more hydrophobic groups on the surface than in the peanut protein isolate (PPI). In vitro, PPA was digested by both pepsin and trypsin more than PPI. FTIR profiles demonstrated there were more β-sheet and less α-helix in PPA, which means much more aggregation. In addition, binding force analysis showed hydrophobic interaction was the major force that restricted dissolution of PPA and disulfide bonds were of second importance, which provided a possible physical treatment opportunity for improving the solubility of PPA. |
