| Pseudomonas aeruginosa菌的原油除蜡降黏性能 |
| |
| 引用本文： | 王卫强,吴尚书,崔静,鲍天宇,张海娟,张政一.Pseudomonas aeruginosa菌的原油除蜡降黏性能[J].石油学报(石油加工),2021,37(1):168-175. |
| |
| 作者姓名： | 王卫强 吴尚书 崔静 鲍天宇 张海娟 张政一 |
| |
| 作者单位： | 1. 辽宁石油化工大学 石油天然气工程学院，辽宁 抚顺 113001；
2. 中国石油 内蒙古鄂尔多斯销售分公司，内蒙古 鄂尔多斯 017000；
3. 辽宁石油化工大学 石油化工学院, 辽宁 抚顺 113001；
3. 中国石油 大庆石化公司，黑龙江 大庆 163000 |
| |
| 基金项目： | 辽宁省高等学校创新人才项目（LR2018055）和辽宁省自然科学基金指导计划项目（20180550745）资助 |
| |
| 摘 要： | 采用单细胞Raman-D2O同位素标记技术确定了5种原油嗜蜡菌中的优势菌种；考察了其最佳生长条件，并对其代谢产物及其对原油的除蜡降黏性能进行测定。结果表明：优势原油嗜蜡菌种为SS1，属于铜绿假单胞菌属(Pseudomonas aeruginosa)；其最佳生长条件为温度36 ℃、pH值7、NaCl质量浓度5 g/L；该菌种在生长代谢过程中产生糖脂类生物表面活性剂，乳化系数高达63.75%；用该菌种对原油处理120 h后，原油蜡降解率为53.16%，析蜡点及析蜡高峰点均有所降低；在36 ℃下，其对原油降黏率达到72.84%。
|
| 关 键 词： | 原油 铜绿假单胞菌属 乳化 蜡降解 降黏 |
| 收稿时间： | 2019-12-19 |
Properties of Pseudomonas Aeruginosa on Wax Removal and Viscosity Reduction in Crude Oil |
| |
| WANG Weiqiang,WU Shangshu,CUI Jing,BAO Tianyu,ZHANG Haijuan,ZHANG Zhengyi.Properties of Pseudomonas Aeruginosa on Wax Removal and Viscosity Reduction in Crude Oil[J].Acta Petrolei Sinica (Petroleum Processing Section),2021,37(1):168-175. |
| |
| Authors: | WANG Weiqiang WU Shangshu CUI Jing BAO Tianyu ZHANG Haijuan ZHANG Zhengyi |
| |
| Affiliation: | 1. College of Petroleum Engineering, Liaoning Shihua University, Fushun 113001, China;
2. Inner Mongolia Ordos Sales Company, CNPC，Ordos 017000, China;
3. College of Petrochemical Engineering, Liaoning Shihua University, Fushun 113001, China;
4. Daqing Petrochemical Company, CNPC，Daqing 163000, China |
| |
| Abstract: | Single-cell Raman-D2O isotope labeling
technique was used to screen out the dominant species from five paraffin-degrading
bacteria. The optimal growth conditions of strain, and their metabolites and
capability to remove wax and reduce viscosity in crude oil were investigated.
The results show that the dominant strain in crude oil paraffin-degrading
bacteria is SS1, which belongs to Pseudomonas
aeruginosa. The optimal growth conditions are the temperature of 36 ℃, the pH value of 7 and
NaCl mass concentration of 5 g/L. The strain produces glycolipid type
biosurfactants, in the process of growth and metabolism, with the emulsification
index of 63.75%. Crude oil was treated with strain SS1 for 120 h, and the rate
of wax degradation is 53.16%, the wax appearance point and the wax appearance
peak point both decrease, and the rate of viscosity reduction reaches 72.84% at
36 ℃. |
| |
| Keywords: | crude oil Pseudomonas aeruginosa emulfification wax degradation viscosity reduction |
|
| 点击此处可从《石油学报(石油加工)》浏览原始摘要信息 |
|
点击此处可从《石油学报(石油加工)》下载全文 |
|
