HPLC法定量分析水中光合细菌菌体产量

为了定量分析水中光合细菌（PSB）菌体产量,为光合细菌污水资源化提供方法依据,采用HPLC法分析CoQ10的质量,进而测得水中PSB的菌体产量.实验结果表明,色谱条件为：色谱柱为SphherisorbC18（10 cm×4.6 mm ID）柱;甲醇与无水乙醇为流动相,体积比9∶1;流速为1 mL/min;柱温35℃;紫...

HPLC quantification of photosynthetic bacteria in water

To test biomass of photosynthetic bacteria(PSB) in water,and to provide a way of PSB sewage reclamation,this paper adopted HPLC to analyze the concentration of CoQ10and thus obtained the concentration of PSB in water.The results show that the chromatographic condition is as follows: The chromatographic column is SphherisorbC18(10 cm×4.6 mm ID);methanol-absolute alcohol(9∶ 1) as mobile phase at a flow rate of 1 mL/min;column temperature 35 ℃;Wave Length of UV detector 275 nm;sample size 15 μL;column pressure 16.4 MPa.By extracting 5 h with acetone,volatilizing acetone completely and dissolving it with absolute alcohol,this method may obtain the maximum relative extraction of CoQ10.The average recovery ratio of external reference method is 99.13% and RSD 0.81%.The highest radiance-extraction is acetone extraction 5 h before evaporating completely and then solving in ethanol.The concentration of CoQ10 in PSB in various cultural conditions and growing phases is quite stable.The impurities or microbes in wastewater do not affect the testing result.The concentration of PSB is(Y+37.487)/25.431 g·L-1,Y is peak area(V·s).This method is simple and accurate,and can be used for quantitative analysis of PSB in water.