| 丁醇高产菌株诱变育种及发酵条件优化研究 |
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| 引用本文： | 王风芹,原欢,谢慧,宋安东.丁醇高产菌株诱变育种及发酵条件优化研究[J].中国酿造,2011(5). |
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| 作者姓名： | 王风芹 原欢 谢慧 宋安东 |
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| 作者单位： | 河南农业大学,生命科学学院,河南,郑州,450002 |
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| 基金项目： | 河南省教育厅自然科学基金 |
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| 摘 要： | 利用低温等离子体和亚硝基胍(NTG)对兼性厌氧产丁醇芽孢杆菌(Bacillus sp.)C2菌株进行复合诱变,分离筛选得到3株丁醇和总溶剂产量均有明显提高的突变菌株,其发酵7%玉米醪液后丁醇和总溶剂产量分别达到12.59g/L、12.44g/L、12.74g/L和20.36g/L、20.14g/L、20.79g/L,比出发菌株分别提高21.6%～24.5%和15.4%～19.1%.对编号为414的突变菌株发酵条件进行优化,在100mL三角瓶发酵体系中,该菌株的最适发酵条件为装液量100mL,种龄24h,接种量10%,pH 7(自然),37℃静置发酵72h,在此条件下,414菌株发酵7%玉米醪液产丁醇和总溶剂量分别达到12.58g/L～13.77g/L和21.25g/L～22.27g/L.
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| 关 键 词： | 低温等离子体 亚硝基胍 复合诱变 丁醇 发酵条件优化 |
Mutation breeding of butanol high-yielding strain and its fermentation condition optimization |
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| WANG Fengqin,YUAN Huan,XIE Hui,SONG Andong.Mutation breeding of butanol high-yielding strain and its fermentation condition optimization[J].China Brewing,2011(5). |
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| Authors: | WANG Fengqin YUAN Huan XIE Hui SONG Andong |
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| Affiliation: | WANG Fengqin,YUAN Huan,XIE Hui,SONG Andong (College of Life Science,Henan Agricultural University,Zhengzhou 450002,China) |
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| Abstract: | Bacillus sp.C2,a facultative anaerobic butanol producing strain,was compound with low temperature plasma and nitrosoguanidine(NTG).Three high yield mutant strains were and their butanol and total solvent yields form 7% corn mash reached 12.59g/L,12.44g/L,12.74g/L and 20.36g/L,20.14g/L,20.79g/L,respectively,which was 21.6%~24.5% and 15.4%~19.1% higher than the original strain.The optimal fermentation conditions of the mutant strain 414 was as following(in 100mL flask fermentation system): 100mL liquid volume... |
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| Keywords: | low temperature plasma nitrosoguanidine compound mutation butanol fermentation condition optimization |
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