A method of freeze-drying and fixing spermatozoa for electron microscopy

A procedure is described whereby freshly ejaculated bull semen is freeze-dried, then treated by glutaraldehyde fixative and processed for electron microscope observation of the spermatozoa. Some of the structural features of bull spermatozoa are demonstrated in electron micrographs. In general the procedure may be found useful as an alternative technique for the examination of such cell-carrying fluids as whole blood, cerebrospinal fluid, as well as bacterial cultures.