QuEChERS-超高效液相色谱-串联质谱法同时测定油料和植物油中77种农药残留量

目的建立油料和植物油中77种农药的QuEChERS-超高效液相色谱串联质谱检测方法。方法采用QuEChERS,用乙腈超声提取,经PSA、C18基质分散净化,以0.1%甲酸/乙腈溶液进行梯度洗脱,采用电离喷雾电离方式(ESI+),通过多反应监测(MRM)定量。结果该法测定时间短,均在10 min内出峰,线性关系良好,相关系数r均达到0.99,回收率在60.2%~99.8%之间,相对标准偏差(RSD)均小于8%。结论该法快速、简便、精确度高、准确度高,对油料及植物油中多类型农药残留具有普遍适用性。

Simultaneous determination of 77 pesticides in oil plants and plant oil by high performance liquid chromatography-tandem mass spectrometry with QuEChERS

Objective To establish a method for the rapid determination of 77 kinds of pesticide residues in oil plants and plant oils by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS). Methods Samples were extracted by QuEChERS with acetonitrile, and purified with PSA+C18 cartridge. A gradient system of a mixture of 0.1% formic acid and acetonitrile with multi-reaction monitoring (MRM) were adopted for quantitative determination. Results Seventy-seven kinds of pesticide residues could be detected within 10 min, the correlation rate (r) was more than 0.99, and the average recoveries ranged from 60.2% to 99.8% with relative standard deviation (RSD) less than 8%. Conclusion This method is rapid, simple, sensitive and accurate, which is suitable for identification and quantification of multiple pesticide residues in oil plants and plant oils.