Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase |
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| Authors: | Y Kinoshita K Nishigaki Y Husimi |
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| Affiliation: | Department of Functional Materials Science, Saitama University, 255 Shimo-okubo, Urawa 338, Japan. |
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| Abstract: | ![]()
A rapid 5'-labeling method of single-stranded DNA/RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5'-ends of both oligo- and polynucleotides. |
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