| 不同发芽条件对蚕豆植酸酶活性的影响 |
| |
| 引用本文： | 罗羽洧,解卫华,马娟,郁志芸.不同发芽条件对蚕豆植酸酶活性的影响[J].中国粮油学报,2012,27(10):32-36,42. |
| |
| 作者姓名： | 罗羽洧 解卫华 马娟 郁志芸 |
| |
| 作者单位： | 1. 金陵科技学院园艺学院,南京,210038
2. 环境保护部南京环境科学研究所,南京,210042 |
| |
| 基金项目： | 江苏省高校自然科学基金(11KJD210001);金陵科技学院博士科研启动基金(jit-b-201205) |
| |
| 摘 要： | 以蚕豆为试材,研究发芽过程中植酸酶在蚕豆不同部位的变化和性质.发芽蚕豆不同部位植酸酶活性呈现先高后低的变化趋势,子叶植酸酶在发芽第6天达最高值.超声波处理对发芽蚕豆胚根、子叶和胚芽植酸酶活性有一定促进作用,其中以15min超声波处理对胚根和胚芽植酸酶活性影响最大,5min处理、10min处理和15min处理均对子叶植酸酶活性有显著影响.在发芽过程中不同光照对蚕豆进行处理,不论胚根、子叶还是胚芽,黑暗处理植酸酶活性高于光照处理.核酸抑制剂放线菌素(Act-D)和蛋白质抑制剂环已酰胺(CHM)对发芽蚕豆胚根、子叶和胚芽中的植酸酶活性有一定影响,其中以CHM的抑制效果最为明显.
|
| 关 键 词： | 蚕豆 发芽 植酸酶 |
Effects of Different Germination Conditions on Phytase Activity on Faba Bean(Vicia faba L.) |
| |
| Luo Yuwei
,
Xie Weihua
,
Ma Juan
,
Yu Zhiyun.Effects of Different Germination Conditions on Phytase Activity on Faba Bean(Vicia faba L.)[J].Journal of the Chinese Cereals and Oils Association,2012,27(10):32-36,42. |
| |
| Authors: | Luo Yuwei Xie Weihua Ma Juan Yu Zhiyun |
| |
| Affiliation: | 1(College of Horticulture,Jinling Institute of Technology1,Nanjing 210038)(Nanjing Institute of Environmental Science of Ministry Environmental Protection2,Nanjing 210042) |
| |
| Abstract: | Phytase activity and property in different faba bean fractions during germination were investigated with faba bean as the testing piece.The phytase activity appeared a change trend of increasing first then decreased with the maximum value at the sixth day during germination in cotyledon.Ultransonic treatments had facilitations onphytase activity in cotyledon,radicle and plumule among which 15 min treatment has the biggest effect on phytase activity in radicle and plumule.5 min treatment,10 min treatment and 15 min treatment had obvious effects on phytase activity in cotyledon.Whether cotyledon,radicle and plumule,faba bean during darkness treatment had higher phytase activity than that of different illuminations during germination.Act-D and CHM had certain effects on cotyledon,radicle and plumule of phytase activity in faba bean,among which,the CHM had the largest inhibition effect on phytase activity. |
| |
| Keywords: | faba bean germination phytase |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
