超声法联合微波辅助提取玉米须甾醇的工艺优化

应用响应面法对超声与微波联合提取玉米须甾醇的工艺进行优化,建立了相应的回归模型。在单因素试验的基础上,考察超声时间、超声波功率、液料比和微波时间对玉米须甾醇得率的影响。通过中心组合试验优化获得最佳工艺条件为:超声时间55 min,超声波功率200W,料液比1:40(g/mL),微波时间16 min,通过五次验证试验,玉米须甾醇的得率为0.76%,与理论预测值基本符合,为玉米须甾醇的开发提供理论依据。

Optimization of Ultrasonic and Microwave Asssisted Extraction Process for Phytosterol from Maize Silk

In order to optimize ultrasonic and microwave assisted extraction process for phytosterol from maize silk, effects of ultrasonic time, ultrasonic power, ratio of solid-liquid and microwave time on phytosterol extraction rate from corn stigma were evaluated. Based on single factor tests, Box-Behnken experimental Design was used and a regression model was established by Response Surface method. Results showed that optimum process conditions were as follows: ultrasonic time 55.00 min, ultrasonic power 200 W, solid-liquid rate 1:40 (g/mL) and microwave time 16 min. Under these conditions, 0.76% of phytosterol extraction rate was obtained. Tested and predicted values for phytosterol extraction rate were similar basically. The results contributed to the development of phytosterol from maize silk.