基于上转换分子印迹聚合物荧光传感检测食品中的章鱼胺

以章鱼胺为模板分子,采用表面分子印迹技术通过溶胶凝胶法在上转换荧光纳米粒子表面合成了对章鱼胺有特异性识别位点的荧光分子印迹聚合物。该聚合物对章鱼胺有良好的特异性吸附和荧光响应。建立了一种适用于食品基质中章鱼胺定量检测的分子印迹荧光传感检测方法,在最佳检测条件下,方法的线性范围为2~10 mg/L,最低检出限为0.37 mg/L,可用于黄酒和乳酪中章鱼胺的检测,回收率86%~98%,相对标准偏差(RSD)0.54%~4.65%。该方法可用于复杂样品中章鱼胺的定量检测。

Fluorescence Sensing Detection of Octopamine in Foods Based on Upconversion Molecularly Imprinted Polymers

In this paper,a fluorescent molecularly imprinted polymers with high selective recognition sites for octopamine was synthesized using octopamine as temple,upconversion fluorescent nanoparticles as matrix and the element of fluorescent sensing by a surface imprinting technique in combination with a sol-gel process.The developed polymers were applied to the quantitative determination of octopamine.Under the optimum conditions,the fluorescence intensity of polymers increased linearly with the increasing concentration of octopamine ranging 2-10 mg/L with a detection limit of 0.37 mg/L(S/N=3).In order to validate the applicability,the fluorescent method was used to detect octopamine in complex samples including wine and cheese.The recoveries ranging 86%-98%with a relative standard deviation(RSD)ranging from 0.54%to 4.65%were obtained.The above result indicated that the proposed fluorescent method provided a new platform for the measurement of octopamine in food samples.