黄芪甲苷抑制黑色素瘤B16细胞的增殖作用

本文研究了黄芪甲苷抑制黑色素瘤B16细胞增殖并诱导细胞凋亡的影响。将细胞培养成功后,分别分成空白对照组、低浓度组(10 mg/mL)、中浓度组(20 mg/mL)、高浓度组(50 mg/mL),检测黄芪甲苷对黑色素瘤B16细胞增殖及凋亡的影响,并测定Caspase-3、B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)、Bax、β-catenin等蛋白的表达量。结果表明,黄芪甲苷具有较好的抗氧化活性,50 mg/mL处理时,其还原能力和DPPH自由基清除能力分别为0.92和70.82%;在此作用浓度下,处理72 h后,其对B16细胞的增殖率和凋亡率分别为15.55%和40.45%,和其他各组均有显著性差异(p<0.05)。此外,在高浓度黄芪甲苷作用下,Caspase-3、Bcl-2、Bax、β-catenin蛋白的表达量分别为0.56、0.58、0.27、0.25,显著区别于其他各实验组(p<0.05),说明黄芪甲苷能抑制较好黑色素瘤B16细胞的增殖,并通过调控Caspase-3、Bcl-2、Bax、β-catenin蛋白表达,诱导细胞凋亡。

Astragaloside IV Inhibits Proliferation and Induces Apoptosis of Melanoma B16 Cells

The effect of astragaloside Ⅳ on the proliferation and apoptosis of melanoma B16 cells was investigated. After the cells were cultured successfully, they were divided into blank control group, low concentration group(10 mg/m L), medium concentration group(20 mg/m L) and high concentration group(50 mg/mL). The effects of astragaloside Ⅳ on proliferation and apoptosis of melanoma B16 cells were evaluated, and the expression levels of caspase-3, B-cell lymphoma-2(Bcl-2), bax and β-catenin were detected. The results showed that astragaloside IV had good antioxidant activity. When treated with 50 mg/mL, the reducing ability and DPPH free radical scavenging capacity were 0.92 and 70.82%, respectively. Under this concentration, the proliferation rate and apoptosis rate of B16 cells after 72 h treatment were 15.55% and 40.45%, respectively, which were significantly different from those of other groups(p<0.05). In addition, the expression levels of caspase-3, Bcl-2, bax and β-catenin were 0.56, 0.58, 0.27 and 0.25, respectively, which were significantly different from those of other groups(p<0.05). Results indicated that astragaloside Ⅳ could inhibit the proliferation of melanoma B16 cells and induce apoptosis by regulating the expression of caspase-3, Bcl-2, bax and β-catenin.