Physicochemical properties of amphotericin B liposomes prepared by reverse-phase evaporation method |
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| Authors: | Rojanapanthu Pleumchitt Sarisuta Narong Chaturon Korakot Kraisintu Krisana |
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| Affiliation: | a Department of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailandb Department of Manufacturing Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailandc Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Nakorn Pathom, Thailandd Research and Development Institute, Government Pharmaceutical Organization, Bangkok, Thailand |
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| Abstract: | The physicochemical properties of phosphatidylcholine-cholesterol liposomes containing amphotericin B and prepared by reverse-phase evaporation method were studied. Uniformly dispersed liposomal suspensions were obtained by employing 3:1 ratio (by volume) of diethyl ether to normal saline, 5 min sonication time at 7°C, and evaporation of diethyl ether at 25°C. Microscopic examination showed that the prepared liposomes were spheroids with unilamellar, oligolamellar, or multilamellar structure. The liposomes containing amphotericin B 2.0 mol% of total lipid led to the highest percentage of drug entrapment. Liposomes with maximum entrapment efficiency were obtained from using 250 µmol of total lipid. The liposomal amphotericin B possessing the highest drug entrapment efficiency (approximately 95%) with particle size range of 1307-1451 nm was the one composed of 1:1 molar ratio of phosphatidylcholine to cholesterol. |
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| Keywords: | Liposomes Amphotericin B Reverse-phase evaporation method Physiochemical properties Oligolamellar vesicles |
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