东北红豆杉中紫杉醇的含量分析 |
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引用本文: | 朱吕会,崔岩,白索柱.东北红豆杉中紫杉醇的含量分析[J].齐齐哈尔轻工业学院学报,2010(4):69-71. |
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作者姓名: | 朱吕会 崔岩 白索柱 |
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作者单位: | [1]齐齐哈尔四友化工有限公司,黑龙江齐齐哈尔161000 [2]齐齐哈尔市第一中学初中部,黑龙江齐齐哈尔161005 |
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摘 要: | 以抗癌活性成分紫杉醇作为标准物,利用高压液相色谱法分析了东北红豆杉树叶和树干的水提取物中抗癌活性成分紫杉醇的含量,确定最佳色谱条件为:色谱柱:C18,Φ4.6×250 mm;流动相:甲醇︰水︰乙腈=45︰35︰20;检测波长:225 nm;柱温:室温;流速:1.0 mL/min。结果表明:东北红豆杉树干中紫杉醇含量高于树叶。
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关 键 词: | 东北红豆杉 紫杉醇 树叶 树干 抗癌 |
Analysis of the contents of Taxol in Taxus cuspidata |
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Authors: | ZHU Lv-hui CUI Yan BAI Suo-zhu |
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Affiliation: | 1.Qiqihar Siyou Chemical Co.,Ltd,Heilongjiang Qiqihar 161000,China;2.Qiqihar No.1 High School,Heilongjiang Qiqihar 161005,China) |
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Abstract: | Taking taxol as a standard sample,a separation condition was established to determine the anti-tumor active taxol content in the extracts of the fresh leaves and barks of taxus.The chromatography conditions were: chromatographic column: C18,Φ 4.6 × 250 mm,flowing solvent: methanol︰water︰acetone nitrile = 45︰35︰20,examination wave length: 225 nm,column warm: room temperature,speed of flow: 1.0 mL/min.The result indicated that the contents of taxol in the bark is higher than that in the leaf. |
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Keywords: | Taxus cuspidata taxol leaf bark anti-tumor |
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