甜菜雄性不育系及保持系花期蛋白差异分析

为揭示甜菜雄性不育的原因,从蛋白质组学角度对甜菜细胞质雄性不育进行研究,利用双向电泳与MALDI-TOF-MS方法,对Owen型甜菜质核互作型雄性不育系DY5-CMS及同型保持系DY5-O花粉发育3个阶段(雄蕊原基分化期、四分体时期和单核时期)的花蕾蛋白差异表达进行研究．结果表明： 在雄蕊原基分化期花蕾中鉴定出6个差异蛋白,在四分体时期鉴定出4个差异蛋白,这些蛋白多数与呼吸和能量代谢有关,推测甜菜细胞质雄性不育可能是花粉发育早期(雄蕊原基分化期和四分体时期)与能量和呼吸代谢有关的蛋白表达上调导致呼吸和能量代谢紊乱造成的;在花粉发育的单核时期鉴定出6个差异蛋白,这些蛋白主要与植物的光合作用有关,推测到花粉发育后期雄性不育性状已形成,雄性不育导致植物光合机能下降．

Proteomic analysis of florescence differentiation between cytoplasmic male sterile line and maintainer in sugar beet

To explore the molecular genetic mechanisms of cytoplasmic male sterility in sugar beet, the differential proteins between the CMS line and its maintainer line were studied. Immobilized pH gradient two-dimensional gel electrophoresis (2-DE) technique and MALDI-TOF-MS were used to identify differentially expressed proteins in the three pollen development stages(stamen primordium differentiation stage, metaphase and uninucleate stage) between the Owen sugar beet male-sterile line(DY5-CMS)and its maintainer line(DY5-O). Six distinct proteins were identified in the stamen primordium differentiation stage and four distinct proteins were identified in the metaphase. In addition, all those proteins were related to the energy and respiratory metabolism. It was inferred that cytoplasmic male sterility of sugar beet might take place at the prophase of pollen development(contain stamen primordium differentiation stage and metaphase). The over-expression of energy and respiratory metabolism related proteins leads to metabolism disorder. Six distinct proteins are identified in the uninucleate stage of pollen development, and most of the proteins are related to photosynthesis of plants. It is speculated that the male sterility trait has formed at the late stages of pollen development and male sterility leads to the decreasing of photosynthetic.