Seasonal and regional variation in triglyceride composition of French butterfat

In the present study, high-performance liquid chromatography analysis of butterfat allowed separation of 46 peaks at 32°C. Knowing the theoretical carbon number value of each triglyceride (TG), 32 peaks of the butterfat chromatogram were identified. These TGs were determined by extrapolation of their capacity factor values, and their identifications were confirmed with some standard TGs. Analysis of winter and summer butterfat from five different French areas showed significant seasonal and regional variation in the TG composition. However, the most important contribution to this variation was provided by TG groups represented by only four peaks. To approximately select the predominant TGs in these four peaks, a random distribution hypothesis was used to predict the amount of each TG. This hypothesis allowed the prediction of the TG components that seem to provide the most important contribution to both seasonal and regional variation.