| Effects of Rare Earth Elements on Promoting Isoflavonoid Production andRelease of Pueraria Lobata Hairy Roots in Vitro |
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| 作者姓名： | 刘传飞 金乐红 刘翠清 |
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| 作者单位： | DepartmentofBasicMedicalSciences,MedicalSchool,HangzhouTeacher＇sCollege,Hangzhou310012,China |
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| 基金项目： | 江西省自然科学基金，杭州师范学院校科研和教改项目 |
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| 摘 要： | The effects of various rare earth elements on growth and isoflavonoid production in hairy root cultures of Pueraria lobata (Willd.) Ohwi (strain TR2) cultured in 500 ml flasks were studied. After 32 days of culture, the biomass of hairy roots increase 15 times and reach 3.2 g dry weight. Hairy root growth was inhibited by Y203, NaSeO3 and Sm^3 be-cause of brown formation. But significant promoting effect on root growth due to callus formation was observed in La^3 treatments. It is the most noteworthy that the production of total isoflavonoids and puerarin was enhanced greatly by La^3 treatment. A major portion of increased total isoflavonoids and puerarin was released into medium in La^3 treatment whilethe hairy root viabilities were preserved. Some specific secondary metabolite release processes could be induced by La^3 and their possible mechanism is discussed.
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| 关 键 词： | 植物学 多毛根栽培 二氢黄酮 稀土元素 组织发育性 |
Effects of Rare Earth Elements on Promoting Isoflavonoid Production and Release of Pueraria Lobata Hairy Roots in Vitro |
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| Abstract: | The effects of various rare earth elements on growth and isoflavonoid production in hairy root cultures of Pueraria lobata(Willd.) Ohwi(strain TR2) cultured in 500 ml flasks were studied. After 32 days of culture,the biomass of hairy roots increase 15 times and reach 3.2 g dry weight. Hairy root growth was inhibited by Y2O3,NaSeO3 and Sm3+ because of brown formation. But significant promoting effect on root growth due to callus formation was observed in La3+ treatments. It is the most noteworthy that the production of total isoflavonoids and puerarin was enhanced greatly by La3+ treatment. A major portion of increased total isoflavonoids and puerarin was released into medium in La3+ treatment while the hairy root viabilities were preserved. Some specific secondary metabolite release processes could be induced by La3+ and their possible mechanism is discussed. |
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| Keywords: | botany pueraria lobata hairy root culture isoflavonoid tissue viability rare earths |
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