反相高效液相色谱法测定人尿中多种多环芳烃代谢物

建立了用反相高效液相色谱-紫外检测法同时检测人尿中6种多环芳烃羟基代谢产物的新方法。采用DiamonsilTMC18(5μm,150 nm×4.6 nm)色谱柱分离,以V(甲醇)∶V(乙酸钠缓冲液)=66∶34为流动相,在波长280 nm检测,流速为0.95 mL/min。结果表明,当9-羟基菲、α-萘酚、β-萘酚、1-羟基芘、2-羟基芴和9-羟基芴的摩尔浓度分别为0.105~500.0μmol/L,0.106~135.0μmol/L,0.120~135.0μmol/L,0.085~50.00μmol/L,0.094~200.0μmol/L和0.101~200.0μmol/L时,峰面积与质量浓度呈很好的线性关系,相对标准偏差分别为2.5%~4.4%,2.1%~4.3%,2.3%~3.2%,1.7%~3.9%,1.9%~2.6%和1.8%~3.5%(n=6),平均回收率分别为92.6%,100.4%,101.0%,91.0%,106.7%和95.5%。方法准确可靠,用于尿样测定,结果满意。

The method for assaying major metabolites of hydroxyl polycyclic aromatic hydrocarbons in human urine by RP-HPLC-UV

A novel method for assaying major metabolites of 6 kinds of polycyclic aromatic hydrocarbons in human urine by RP-HPLC-UV were established. These were detected at 280 nm and separated on Dia-monsil TM C18（5 μm,150 nm × 4. 6 nm）by using sodium acetate buffer solution（66： 34,V： V）as mobile phase when the flow rate was 0. 95 mL/ min. The result showed that the calibration curves of 9-hydroxy-phenanthrene,α-naphthol,β-naphthol,1-hydroxypyrene,2-hydroxyfluorene and 9-hydroxyfluorene showed good linearities in the ranges of 0. 105 ~ 500. 0 μmol/ L,0. 106 ~ 135. 0 μmol/ L,0. 120 ~135. 0 μmol/ L,0. 085 ~ 50. 0 μmol/ L,0. 094 ~ 200. 0 μmol/ L and 0. 101 ~ 200. 0 μmol/ L,respective-ly. The relative standard deviations（RSD）were 2. 5% ~ 4. 4% ,2. 1% ~ 4. 3% ,2. 3% ~ 3. 2% ,1. 7% ~3. 9% ,1. 9% ~ 2. 6% ,1. 8% ~ 3. 5%（ n = 6）and the average recoveries were 92. 6% ,100. 4% , 101. 0% ,91. 0% ,106. 7% and 95. 5% ,respectively. The proposed method was accurate and suitable for the identification and quantification of these compositions in human urine.