基于响应面法和熵权法优化连翘叶中连翘酯苷A、连翘苷和芦丁提取工艺

本文优化了连翘叶多指标成分的超声提取工艺。采用高效液相色谱法（High Performance Liquid Chromatography，HPLC）同时测定连翘叶提取物中连翘酯苷A、连翘苷和芦丁的提取量，以乙醇浓度、液料比、提取温度及提取时间为考察因素，运用熵权法计算权重系数，进行多指标综合评分，并以综合评分为指标，通过Box-Behnken响应面法优化提取工艺。结果表明，连翘叶最佳提取工艺:乙醇浓度50%，液料比28:1 mL/g，提取温度51 ℃，提取时间25 min。验证实验表明在此优化工艺条件下，连翘酯苷A、连翘苷和芦丁的提取量分别为69.69、22.87、22.46 mg/g，综合评分为32.18，RSD均小于3%。该提取工艺稳定、可行，有效成分提取量较高，适用于连翘叶连翘酯苷A、连翘苷和芦丁的提取。

Optimization of Extraction Process of Forsythioside A,Forsythin and Rutin from Forsythia suspensa Leaves Based on Response Surface Methodology and Entropy Weight Method

In this paper, the ultrasonic extraction technology of Forsythia suspensa leaves was optimized based on multi-index components. The contents of forsythioside A, forsythin and rutin were determined by high performance liquid chromatography (HPLC). The ethanol concentration, liquid-solid ratio, extraction time and extraction temperature were taken as the factors. The entropy weight method was used to obtain comprehensive score by calculating the weight coefficient of each index. Subsequently, the comprehensive score was taken as the index. Finally the Box-Behnken response surface methodology was adopted to optimize the extraction process. The optimum extraction conditions of Forsythia suspensa leaves were as follow: Ethanol concentration 50%, liquid to material ratio 28:1 mL/g, extraction temperature 51 ℃, extraction time 25 min. The results of the validation tests showed that under the optimal conditions, the extraction amount of forsythioside A, forsythin and rutin were 69.69, 22.87 and 22.46 mg/g, respectively, the RSD was less than 3% and comprehensive score was 32.18. The extraction technology was stable and feasible, and the extraction quantity of active components was high. It was suitable for the extraction of Forsythia suspensa leaves.