基于保留指数的草莓中杀菌剂快速测定方法建立

基于保留指数原理，利用正构烷烃C₉~C₃₃标准溶液和智能农药数据库（Smart Database Pesticides），建立草莓中32种杀菌剂的快速分析方法。采用QuEChERS前处理方法，对草莓中杀菌剂残留进行提取、净化，使用气相色谱-串联质谱（GC-MS/MS）仪进行定性、定量检测。结果表明，32种杀菌剂在5~200 μg/L范围内线性关系良好，相关系数均大于0.9982，在10、50、100 μg/kg三个添加水平下，加标回收率在70.3%~117.8%之间，相对标准偏差在0.01%~8.50%之间，方法的检出限为0.01~2.91 μg/kg，定量限为0.04~9.71 μg/kg。该方法快捷、简便、准确、稳定，在草莓样品的分析中取得了较好的效果，对监测草莓中杀菌剂的使用情况，同时测定多组分农药残留提供了可靠有效的数据和技术支持。

Establishment of Method for the Rapid Determination of Fungicides in Strawberry Based on Retention Index

C₉~C₃₃ n-alkane standard solution and Smart Database Pesticides database were used to establish a rapid analytical method for the determination of 32 fungicides in strawberry based on retention index. Thirty-two fungicides were selected as the target, the samples were extracted and purified by QuEChERS method. Gas chromatography tandem mass spectrometry was used for qualitative and quantitative analysis. Results showed that, the 32 fungicides showed good linear relationship in the range of 5~200 μg/L, and the correlation coefficients were greater than 0.9982. The recoveries of 32 fungicides spiked at 10, 50, 100 μg/kg were 70.3%~117.8% and therelative standard deviations were 0.01%~8.50%. The limits of detection were 0.01~2.91 μg/kg, and the limits of quantitative were 0.04~9.71 μg/kg. This method was rapid, simple, accurate, steady, and achieved good performance in strawberry sample analyses, and was suitable for monitoring fungicides using in strawberry, and provided reliable date and technical support for determination of multi-component pesticide residues studies．