The use of “lab-on-a-chip” microfluidic SDS electrophoresis technology for the separation and quantification of milk proteins |
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| Authors: | Skelte G. Anema |
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| Affiliation: | 1. Chemisches und Veterinäruntersuchungsamt (CVUA) Karlsruhe, Weissenburger Strasse 3, 76187 Karlsruhe, Germany;2. Bruker Biospin GmbH, Silberstreifen 4, 76287 Rheinstetten, Germany;3. Institute of Chemistry, Saratov State University, Astrakhanskaya Street 83, 410012 Saratov, Russia;4. Spectral Service, Emil-Hoffmann-Strasse 33, 50996 Cologne, Germany;5. Eurofins Analytik GmbH, Neulaender Kamp 1, 21079 Hamburg, Germany;6. Eurofins Analytics France, Rue Pierre Adolphe Bobierre 9, Nantes, France;1. Department of Food and Nutritional Sciences, University of Reading, Reading, UK;2. UCD Institute of Food and Health, School of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland;1. Department of Chemical Sciences, University of Padova, Via Marzolo 1, 35131 Padova, Italy;2. Dipartimento di Scienze Chimiche e Farmaceutiche, Università degli Studi di Trieste, Via L. Giorgieri 1, 34127 Trieste, Italy;3. illycaffè S.p.A., Via Flavia 110, 34147 Trieste, Italy;1. Wadsworth Center, New York State Department of Health, Department of Environmental Health Sciences, School of Public Health, State University of New York at Albany, Empire State Plaza, P.O. Box 509, Albany, New York 12201-0509, USA;2. Department of Chemistry, University of La Rioja, C/Madre de Dios 51, E-26006 Logroño, La Rioja, Spain;3. Biochemistry Department, Faculty of Science and Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia |
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| Abstract: | The use of a microfluidic “lab-on-a-chip” technique for the separation and quantification of milk proteins is described and compared with traditional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The microfluidic chip technique could separate all major milk proteins when standard protein solutions were used. In a milk system, α-lactalbumin, β-lactoglobulin, αs-casein, β-casein and κ-casein were readily separated; the resolution was comparable with SDS-PAGE. However, the immunoglobulins, lactoferrin and bovine serum albumin could not be resolved from the background in the microfluidic chip technique, but were easily resolved by SDS-PAGE. Standard curves for the major whey proteins were linear with both techniques and the calculated concentrations of the major proteins in a milk sample were comparable using both microfluidic chip and SDS-PAGE techniques. These results indicate that the microfluidic chip technology may be a rapid alternative for the separation and quantification of proteins in milk protein based systems. |
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