两种晶型二氧化钛对神经细胞生长的影响

为了研究二氧化钛在免疫隔离技术中的应用,采用溶胶-凝胶法在不同烧结温度下,以多孔钛为基底制备出金红石和锐钛矿两种不同晶型的二氧化钛(TiO_2)膜,并将大鼠下丘脑神经细胞接种到TiO_2膜上,并通过体外培养观察这两种晶型的TiO_2对神经细胞生长的影响.实验结果显示,在金红石型TiO_2膜上生长的神经细胞不仅具有典型的神经细胞结构,而且能正常分泌β-内啡肽,使细胞外液中的β-内啡肽含量始终维持在42.5～45.0 pmol/L;体外培养的第7天,神经细胞经台盼蓝染色检测其存活率为86.7%,并且细胞突起交错成网;但是在锐钛矿型TiO_2膜上生长的下丘脑神经细胞则多数死亡,其存活率仅为5.3%,而少量存活的细胞不仅没有典型的神经细胞结构,而且逐渐丧失了分泌内啡肽的功能.对比结果显示金红石型TiO_2比锐钛矿型TiO_2更有利于神经细胞的生长.

Effect of TiO_2 membranes with different phase composition on growth of neurons

The Effect of TiO_2 membranes with different phase composition on the adherent cells growth were observed by hypothalamic neurons culture in vitro for the immunoisolation application of TiO_2 membranes. The TiO_2 membranes with different phase composition supported on porous Ti planar were prepared under different sintering temperature by sol - gel technique. The results showed that the hypothalamic neurons could grow well on the TiO_2 membranes with rutile phase. These neurons not only had typical nerval morphological characters,but also kept its secretory function of β - endorphin. The contents of β - endorphin remained between 42. 5 pmol/L and 45.0 pmol/L. The livability of neurons, which were cultured on the TiO_2 with rutile phase, was 86.7%. At the same time, the hypothalamic neurons, which were cultured on the TiO_2 membranes with anatase phase, mostly died and the surviving neurons (5.3%) had neither normal nerval morphological charac-ters nor secretory function. These results indicated that the TiO_2 membranes with rutile phase were more propitious to adherent cells growth than that with rutile phase.