Characterization and osteoblast-like cell compatibility of porous scaffolds: bovine hydroxyapatite and novel hydroxyapatite artificial bone |
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Authors: | Yuan Gao Wen-Ling Cao Xiao-Yan Wang Yan-Dao Gong Jie-Mo Tian Nan-Ming Zhao Xiu-Fang Zhang |
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Affiliation: | (1) Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, 100084, China;(2) Institute of Nuclear Energy Technology, Tsinghua University, Beijing, 100084, China |
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Abstract: | Three different porous scaffolds were tested. The first two were prepared by sintering bovine bone. The third scaffold was
prepared using three-dimensional gel-lamination, a new rapid prototyping method, and was named as hydroxyapatite artificial
bone.
X-ray diffraction and Fourier transform infrared spectroscopy analysis confirmed that the samples were mainly highly crystalline
hydroxyapatite ceramics. Scanning electron microscopy and mercury intrusion porosimetry measurement showed that the pores
were interconnected and pore sizes ranged from several microns to hundreds of microns.
Mouse osteoblast-like cells grown on the three scaffolds retained their characteristic morphology. Cell proliferation and
differentiation, analyzed by methylthiazol tetrazolium (MTT) and alkaline phosphatase activity assays, were significantly
higher on the hydroxyapatite artificial bone than on the other two scaffolds tested. All the scaffolds provided good attachment,
proliferation and differentiation of bone cells.
These results indicate that the scaffolds have a favorable interaction with cells, they support cell growth and functions,
and therefore these scaffolds may have great potential as bone substitutes. The three-dimensional gel-lamination method is
proven to be an attractive process to design and fabricate bone scaffolds with favorable properties, and therefore, has promising
potential for bone repair applications. |
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