虾青素调控ROS介导的自噬延长秀丽隐杆线虫寿命作用

本文研究了虾青素(Astaxanthin,AST)延长秀丽隐杆线虫寿命及其相关分子机制.观察AST对野生型(WT)线虫的寿命的影响,qRT-PCR检测WT线虫抗氧化(sod-3、ctl-1)和自噬相关基因(bec-1、lgg-1)的相对表达量,DCFH-DA荧光探针检测AST对WT线虫体内ROS (Reactive o...

Astaxanthin Regulates ROS-mediated Autophagy to Prolong Lifespan of Caenorhabditis elegans

The present study was carried on assess the lifespan extension of Caenorhabditis elegans(C.elegans)by astaxanthin(AST)and its related molecular mechanisms.The effect of AST on the lifespan of wild-type(WT)nematodes was observed,the relative expression of antioxidant(sod-3,ctl-1)and autophagy-related genes(bec-1,lgg-1)in WT nematodes was detected by qRT-PCR,the effect of AST on ROS levels in WT nematodes was detected by DCFH-DA fluorescent probe,the effect of AST on LGG-1::GFP fluorescent protein expression in DA2123 nematodes was detected by Western blot,and after oxidative stress was induced by juglone,the mRNA expression levels of bec-1 and lgg-1 in WT nematodes under oxidative stress were detected by qRT-PCR,and the effect of AST on the lifespan of C.elegans under oxidative stress was further examined.The results showed that AST could prolong the lifespan of WT nematodes,upregulate the mRNA expression levels of sod-3,ctl-1,bec-1,and lgg-1 genes,scavenge excessive ROS in nematodes,upregulate autophagy levels and prolong nematode lifespan under oxidative stress.Therefore,AST might prolong nematode lifespan by regulating ROS levels in C.elegans and then regulating autophagy.