| 木聚糖酶TgXyn2的酶学特性研究 |
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| 引用本文： | 葛雨杭,陈美汐,孙博,吴松娜,万群.木聚糖酶TgXyn2的酶学特性研究[J].食品工业科技,2022,43(4):138-144. |
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| 作者姓名： | 葛雨杭 陈美汐 孙博 吴松娜 万群 |
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| 作者单位： | 1.南京农业大学食品科技学院，江苏南京 210095)2.南京农业大学理学院，江苏南京 210095 |
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| 基金项目： | 南京农业大学2020年校级创新训练立项项目(202018XX09);国家自然科学基金面上项目(31670790)。 |
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| 摘 要： | 目的:本研究旨在发掘和研究一种新的低温酸性内切- 1,4 -β-木聚糖酶TgXyn2并研究其酶学特性，为其在食品等轻工业生产中的实际应用提供理论依据。方法:利用pCold-TF表达质粒在大肠杆菌中异源表达，纯化后检测其酶学特性，并通过同源建模分析其三维结构。结果:该酶的最适温度为35 ℃，最适pH为5.0，Km为1.287 μmol L?1，Vmax为2.083 μmol min?1 mg?1；同源建模结果表明，TgXyn2由2个反向平行的β-折叠和1个α-螺旋构成，是典型的糖苷水解酶GH11家族木聚糖酶。结论:本文研究的TgXyn2具有良好的酶学特性，在食品等行业具有较好的应用潜力。
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| 关 键 词： | 贵州木霉 木聚糖酶 酶学特性 第11家族 食品行业 |
| 收稿时间： | 2021-06-17 |
Enzymatic Characterization of Xylanase TgXyn2 |
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| GE Yuhang,CHEN Meixi,SUN Bo,WU Songna,WAN Qun.Enzymatic Characterization of Xylanase TgXyn2[J].Science and Technology of Food Industry,2022,43(4):138-144. |
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| Authors: | GE Yuhang CHEN Meixi SUN Bo WU Songna WAN Qun |
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| Affiliation: | 1.School of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China2.College of Science, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | Objectives: This study aimed to discover a acidic xylanase TgXyn2 with high enzymatic activities at low temperatures, which might have potential application in food industry. Method: TgXyn2 was heterologously expressed in E.coli with the expression vector pCold-TF. Results: The optimum reaction condition of TgXyn2 was 35 ℃ and pH5.0, respectively. Its Km was 1.287 μmol L?1 and Vmax was 2.083 μmol min?1 mg?1. Homology modeling showed that TgXyn2 had two antiparallel β-sheets and an α-helix, which was typical for the GH11 family. Conclusions: TgXyn2 had good enzymatic activities, which has potential application in food industry. |
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| Keywords: | Trichoderma guizhouense xylanase enzymatic activity GH11 food industry |
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