Development of a method for direct quantification of cytomegalovirus antigenemia by flow cytometry |
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| Authors: | BM Imbert-Marcille N Robillard AS Poirier M Coste-Burel D Cantarovich N Milpied S Billaudel |
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| Affiliation: | Virology Laboratory, Institute of Biology of Nantes University Hospital, France. bmimbert@sante.univ-nantes.fr |
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| Abstract: | ![]()
Cytomegalovirus (CMV) antigenemia was directly detected in polymorphonuclear leukocytes (PMNLs) from transplant recipients by using flow cytometry (FC). Two fixation and permeabilization methods and seven anti-CMV monoclonal antibodies (MAbs) were evaluated. 1C3, SL20, and NEA-9221 MAbs were more efficacious. The antigenemia detection threshold of FC was 0.05% positive PMNLs, and percentages correlated well with DNA viral load and the appearance of clinical symptoms. |
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