Investigation of polysaccharides by two-dimensional N.M.R. Spectroscopy

Summary This paper is relative to a study of polysaccharides in solution by two-dimensional nuclear Overhauser enhancement (2D NOE) and correlated (SECSY) experiments. The main advantages and limitations of these techniques are described in the case of molecules in solution into dimethyl sulfoxide (d.m.s.o.). In particular only one 2D NOE experiment is capable to provide informations concerning dipolar interactions as well as chemical exchange phenomena. It also open a new route to obtain conformational data with polysaccharides.Laboratoire Propre du C.N.R.S., Associé à l'Université Scientifique et Médicale de Grenoble