乳酸菌UV_3-9包埋固定化技术发酵产共轭亚油酸

采用包埋法将乳酸菌UV3-9固定化处理后,对该固定化乳酸菌小球进行了发酵生产共轭亚油酸(CLA)的研究。结果显示,固定化小球的最佳直径为2～3 mm,固定化小球在氯化钙和硼酸混合液中需浸泡过夜;固定后的小球发酵产CLA最佳条件为:发酵温度37℃,初始pH 6.5,发酵时间28 h,底物LA添加量为0.3%,CLA最大产量达到92.061μg/mL。该固定化乳酸菌小球已完成了7批次的共轭亚油酸发酵,其产量均在80.476μg/mL以上。而且固定化UV3-9的CLA发酵产量是游离UV3-9的1.28倍。

Fermentation of Conjugated Linoleic Using Lactic Acid Bacteria Acid Through Embedding Immobilization Technology

This paper focused on the fermentation of conjugated linoleic acid(CLA) using immobilized lactic acid bacteria through embedding method.The results showed that the best diameter of immobilized ball was 2~3 mm.Furthermore,immobilized ball should be immersed in the mixture of calcium chloride and boric acid overnight.The best ferment conditions of immobilized ball were as follows: fermentation temperature of 37 ℃,initial pH of 6.5,fermentation time of 28 h,the substrate adding amount of 0.3 %,and maximum yield of CLA reached 92.061 μg/mL.7 batches of conjugated linoleic acid fermentation have been carried out using the immobilized lactic acid bacteria ball,and their outputs were higher than 80.476 μg/mL.And fermentation production of CLA using immobilized UV3-9 was 1.28 times of which using free UV3-9.