In vitro selection of aptamers that bind to ribosome-inactivating toxins |
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Authors: | I Hirao S Yoshinari S Yokoyama Y Endo AD Ellington |
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Affiliation: | Yokoyama CytoLogic Project, ERATO, Saitama, Japan. |
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Abstract: | Ribosome-inactivating proteins, such as ricin, pepocin and gypsophilin, catalyze the hydrolysis of a single N-glycosidic bond at a specific position in rRNAs. Aptamers targeting pepocin were selected from a random sequence RNA pool that spanned 30 positions. After 8 rounds, the anti-pepocin aptamers were sequenced and a conserved hairpin motif was identified. Interestingly, the selected motif is quite different from the toxin-binding domains of rRNAs. |
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