碱性蛋白酶水解红曲霉菌体的研究

为了使红曲霉菌体滤渣得到高值化利用,对红曲霉菌体的酶解条件进行研究。采用碱性蛋白酶对红曲霉菌体进行酶解,通过单因素试验和正交试验,确定最佳的酶解条件:酶解pH为8.5,酶解温度为55℃,底物浓度为40g/L,酶量为6×104 U/g.干菌体,酶解时间为20h。该条件下,酶解率为25.28%。

Study on optimization of process conditions for enzymatic hydrolysis of cell protein of monascus with alkaline protease

To achieve more valuable utilization of cell filtered sediment of Monascus,the process conditions for enzymatic hydrolysis of cell protein of Monascus with alkaline protease was studied.Using the single factor and orthogonal design experiment,respectively,the optimum conditions of enzymatic hydrolysis were determined as follows: pH of 8.5,temperature of 55 ℃,concentration of substrate of 40 g/L,enzyme dosage of 6×104 U/g(cell amount),and enzymatic hydrolysis time of 20 hours.Under above optimum conditions,the enzymatic hydrolysis rate was 25.28%.