不同品种杭白菊中酚类物质含量和清除自由基活性的比较

4种酮乡产杭白菊及黄菊和野菊，分别用乙醇-水体系进行热回流提取，用比色法测定醇提物中总酚和总黄酮含量，并用化学发光法检测春清除活性氧自由基的能力。结果表明，不同品种间含量存在较大差异，总酚和总黄酮含量（以菊花干基计）最高提异种大白菊，分别为9.76%和4.46%；最低的是黄山种野菊，分别为3.21%和1.99%。不同菊花均显示了良好的清除活性氧自由基的活性，清.OH和O2^.-的IC50分别在0.54-1.98mg/ml和24.2-54.5ug/ml之间，其中清O2^.-活性最强是大洋菊，最弱是软梗小洋菊；而清.OH能力最强为早小洋菊，最终为异种大白菊，结果表明，菊花中酚类物质的含量高低与抗自由基活性强弱无明显的对应关系。

Studies on Phenolic Compound Content and Anti-free Radical Capacity of Several Chrgsanthemums

Six species chrysanthemum samples, including4 Hangbaiju, 1 Huangju and 1 Yeju, were collected fromthe famous "Hangbaiju Kingdom of China", Tongxiang city, Zhejiang province. Fresh flower was dried by microwave oven assisted by hot air circulation, and extracted individually by thermal reflux with 70％ ethanol - water solution. The contents of total phenols (TP) and total flavonoids (TF) of these extracts were measured by spectrophotometric analysis. Obvious differences were found in these chrysanthemums: Yizhongdabaiju had the highest contents at 9. 76％ TP and 4. 46％ TF (in dry base of flower), and Yeju was the lowest one at 3.21％ TPand 1.99％ TF. Chemiluminescence method demonstrated that all of samples had good scavenging capacity on reactive oxygen free radicals. The IC 50 ranged in 0.54 ～ 1.98mg/ml to · OH and 24. 2 ～ 54. 5μg/ml to O 2 -, respectively. Among these chrysanthemums,Dayangju had the strongest scavenging activity to O 2 -, and Ruangengxiaoyangju was the weakest one; similarly, Zaoxiaoyangju had the strongest scavenging activity to · OH and Yizhongdabaiju was the weakest one. This research showed no obvious correlativity between the phenolic compound content of chrysanthemums and their anti - free radical capacity.