| 全细胞脂肪酸气相色谱法鉴别乳杆菌和明串珠菌 |
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| 引用本文： | 陈锡赉,王荣民,顾以林,沈国惠,汤丹剑.全细胞脂肪酸气相色谱法鉴别乳杆菌和明串珠菌[J].食品与生物技术学报,1993,12(1). |
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| 作者姓名： | 陈锡赉 王荣民 顾以林 沈国惠 汤丹剑 |
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| 作者单位： | 无锡轻工业学院食品科学与工程系
(陈锡赉，王荣民，顾以林，沈国惠)，无锡轻工业学院食品科学与工程系(汤丹剑) |
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| 摘 要： | 运用气相色谱—质谱联用技术对6种乳杆菌(14株)和3种明串珠菌(3株)进行全细胞脂肪酸组成分析。根据细胞脂肪酸组成和特征峰的峰形可以把测试菌株分为5群。用这方法9株中只有3株的菌种得到鉴别。而用微机对脂肪酸组成进行聚类分析,可使除干酪乳杆菌1042(不典型菌株)和阿拉伯聚糖乳杆菌以外的所有测试菌株均能鉴别到种的水平。
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| 关 键 词： | 全细胞脂肪酸 乳杆菌 明串珠菌 聚类分析 |
Gas Chromatography of Whole-cell Fatty Acid in the Discrimination of lactobaciili and Leuconstocs |
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| Chen Xielai Wang Rongmin Gu Yeling Shen Guohuai Tong Danjian.Gas Chromatography of Whole-cell Fatty Acid in the Discrimination of lactobaciili and Leuconstocs[J].Journal of Food Science and Biotechnology,1993,12(1). |
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| Authors: | Chen Xielai Wang Rongmin Gu Yeling Shen Guohuai Tong Danjian |
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| Affiliation: | Dept. of Food Science and Eng. |
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| Abstract: | The whole-cell fatty acids of 6 species (14 strains), of Lactobacilli and 3 species (3 starins) of Leticonostocs were analyzed by gas chromatography-mass spectrometric method. Based on the cellular fatty acid components and the forms of the characteristic peaks, all examined strains could be divided into five groups, by means of which only 3 of 9 species examined were found to be distinguishable. But all the examined strains except Lactobacillus casei 1042 (atypical strain) and Lactobacillus arabinosus were able to be dis-criminated to the level of species based on cellular fatty acids analsys by hierachical clus-tering analysis with microcomputer. |
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| Keywords: | Whole-cell fatty acids lactobacillus Leuconostoc Clustering analysis |
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