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双波长分光光度法测定金柚皮中总黄酮的含量
引用本文:朱远平,牟利辉.双波长分光光度法测定金柚皮中总黄酮的含量[J].广东化工,2007,34(12):90-92,99.
作者姓名:朱远平  牟利辉
作者单位:嘉应学院,生物系,广东,梅州,514015
摘    要:以乙醇为提取剂,微波辅助提取法提取金柚皮中的总黄酮。应用双波长分光光度法,测定金柚皮中的总黄酮含量,测定波长510 nm,参比波长588 nm,平均回收率99.32%,RSD=0.64%(n=5)。结果表明,梅州产金柚的柚皮中总黄酮平均含量达1.0%以上,该方法稳定可靠,重复性好。

关 键 词:金柚  金柚皮  总黄酮  双波长分光光度法
收稿时间:2007-09-04
修稿时间:2007年9月4日

Determination of Total Flavonoids in Golden-pomelo Peels By Double-wavelength Spectrophotometry
Zhu Yuanping,Mou Lihui.Determination of Total Flavonoids in Golden-pomelo Peels By Double-wavelength Spectrophotometry[J].Guangdong Chemical Industry,2007,34(12):90-92,99.
Authors:Zhu Yuanping  Mou Lihui
Abstract:The total flavonoids in golden-pomelo peels was extracted by microwave-asisted extraction using ethanol as solvent.A double-wavelength spectrophotometry was applied to determine the total flavonoids in golden-pomelo peels.The detective wavelength was 510 nm,while the reference one was 588 nm,the recovery was 99.32%,RSD=0.64%(n=5).The result indicated the average total flavonoids content in golden-pomelo peels from Meizhou was above 1.0%,the method was stable,reliable and accurate in the determining process.
Keywords:golden-pomelo  golden-pomelo peels  total flavonoids  double-wavelength spectrophotometry
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