| 用HPLC法测定国内反应香精中的4,8-DiMeIQx和PhIP |
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| 引用本文： | 崔俭杰,李琼,金其璋.用HPLC法测定国内反应香精中的4,8-DiMeIQx和PhIP[J].化学世界,2010,51(2). |
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| 作者姓名： | 崔俭杰 李琼 金其璋 |
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| 作者单位： | 1. 上海应用技术学院,上海,200235;上海香料研究所,上海,200232
2. 上海香料研究所,上海,200232 |
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| 基金项目： | 上海市教委基金项目资助(05D205009) |
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| 摘 要： | 建立同时测定反应香精中2-胺基-3,4,8-三甲基-3H-咪唑并4,5-F]喹喔啉(4,8-DiMeIQx)和2-胺基-1-甲基-6-苯基咪唑4,5-b]嘧啶(PhIP)的高效液相色谱(HPLC)分析方法。用强酸水解反应香精,在碱性环境下用二氯甲烷萃取分析物,弱酸净化。以乙腈和pH3.44的醋酸-醋酸胺缓冲溶液梯度洗脱,264 nm(测定4,8-DiMeIQx)和312 nm(测定PhIP)双波长扫描,进行高效液相色谱检测。4,8-DiMeIQx和PhIP在反应香精中的检出限均为0.01 mg/L;加标回收率分别为87.25%和102%。用该法对国内不同公司的不同产品进行检验适用性良好。
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| 关 键 词： | 反应香精 杂环胺 4 8-DiMeIQx PhIP 高效液相色谱法 |
Determination of 4,8-DiMeIQx and PhIP in Domestic Process Flavor by HPLC |
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| CUI Jian-jie,LI Qiong,JIN Qi-zhang.Determination of 4,8-DiMeIQx and PhIP in Domestic Process Flavor by HPLC[J].Chemical World,2010,51(2). |
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| Authors: | CUI Jian-jie LI Qiong JIN Qi-zhang |
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| Affiliation: | CUI Jian-jie~,LI Qiong~ |
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| Abstract: | A high-performance liquid chromatography(HPLC) analytical method for determination of 2-amino-1-methyl-6-phenylimidazo pyridine(PhIP) and 2-amino-3,4,8-trimethyl-3H-imidazo quino xaline (4,8-DiMeIQx) in process flavor was estalliohed.The process flavor samples were hydrolyzed by a strong acid.The sample was extracted from the solution after treatment by dichloromethane in alkaline environment,and then purified by a weak acid.Acetonitrile and pH3.44 acetic acid-ammonium acetate buffer solution were used as t... |
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| Keywords: | 4 8-DiMeIQx PhIP process flavor heterocyelic amines 2-amino-3 4 8-trimethyl-3H-imidazo[4 5-F] quino xaline (4 8-DiMeIQx) 2-amino-1-methyl-6-phenylimidazo [4 5-b]pyridine(PhlP) HPLC |
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